The role of Mig1, Mig2, Tup1 and Hap4 transcription factors in regulation of xylose and glucose fermentation in the thermotolerant yeast Ogataea polymorpha

被引:12
作者
Kurylenko, Olena [1 ]
Ruchala, Justyna [1 ,2 ]
Kruk, Barbara [2 ]
Vasylyshyn, Roksolana [1 ]
Szczepaniak, Justyna [2 ]
Dmytruk, Kostyantyn [1 ]
Sibirny, Andriy [1 ,2 ]
机构
[1] NAS Ukraine, Dept Mol Genet & Biotechnol, Inst Cell Biol, Drahomanov St,14-16, UA-79005 Lvov, Ukraine
[2] Univ Rzeszow, Dept Microbiol & Mol Genet, Cwiklinskiej 2D,Bldg D10, PL-35601 Rzeszow, Poland
关键词
transcriptional factors; xylose; glucose; non-conventional yeasts; Ogataea polymorpha; HANSENULA-POLYMORPHA; SACCHAROMYCES-CEREVISIAE; REPRESSION; METABOLISM; CONTAINS; ETHANOL; GENE; OVEREXPRESSION; INTEGRATION; EXPRESSION;
D O I
10.1093/femsyr/foab029
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Glucose is a preferred carbon source for most living organisms. The metabolism and regulation of glucose utilization are well studied mostly for Saccharomyces cerevisiae. Xylose is the main pentose sugar released from the lignocellulosic biomass, which has a high potential as a renewable feedstock for bioethanol production. The thermotolerant yeast Ogataea (Hansenula) polymorpha, in contrast to S. cerevisiae, is able to metabolize and ferment not only glucose but also xylose. However, in non-conventional yeasts, the regulation of glucose and xylose metabolism remains poorly understood. In this study, we characterize the role of transcriptional factors Mig1, Mig2, Tup1 and Hap4 in the natural xylose-fermenting yeast O. polymorpha. The deletion of MIG1 had no significant influence on ethanol production either from xylose or glucose, however the deletion of both MIG1 and MIG2 reduced the amount of ethanol produced from these sugars. The deletion of HAP4-A and TUP1 genes resulted in increased ethanol production from xylose. Inversely, the overexpression of HAP4-A and TUP1 genes reduced ethanol production during xylose alcoholic fermentation. Thus, HAP4-A and TUP1 are involved in repression of xylose metabolism and fermentation in yeast O. polymorpha and their deletion could be a viable strategy to improve ethanol production from this pentose.
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页数:12
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