Fibrosis and matrix metalloproteinases in rat renal allografts

被引：37

作者：

Inkinen, KA

Soots, AP

Krogerus, LA

Lautenschlager, IT

Ahonen, JP

机构：

[1] Univ Helsinki Hosp, Transplant Unit, Res Lab, Dept Surg, FIN-00029 Helsinki, Finland

[2] Univ Helsinki Hosp, Dept Virol, FIN-00029 Helsinki, Finland

[3] Univ Helsinki Hosp, Dept Pathol, FIN-00029 Helsinki, Finland

[4] Univ Helsinki, Helsinki, Finland

来源：

TRANSPLANT INTERNATIONAL | 2005年 / 18卷 / 05期

关键词：

chronic rejection; collagens; fibrosis; kidney transplantation; matrix metalloproteinases; tissue inhibitors of matrix metalloproteinases;

D O I：

10.1111/j.1432-2277.2004.00053.x

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

The temporal activity and gene expression of matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinase (TIMP) were investigated in a rat model of chronic allograft nephropathy. Gelatinolytic activity of MMP-2 and -9 were demonstrated by zymography, and MMP-2,-9 and TIMP-3 mRNA by in situ hybridization. The generation of fibrosis was determined as total collagen content/DNA. Significantly more latent and active MMP-2, as well as latent MMP-9, were seen in allografts than in autografts. Intense MMP-2 mRNA expression was demonstrated in the allografts during the first 20 days after transplantation, located mainly in the interstitium of the kidney. In addition,some tubular cells expressed MMP-2 mRNA. After day 20, MMP-2 gene expression was faint. MMP-9 mRNA expression in allografts was located mainly in the glomerulus. TIMP-3 mRNA expression was downregulated in allografts. MMP-2, MMP-9 and TIMP-3 seem to play a critical role in the development of fibrosis in the renal allograft.

引用

页码：506 / 512

页数：7

共 29 条

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