RETRACTED: Hesperidin exhibits in vitro and in vivo antitumor effects in human osteosarcoma MG-63 cells and xenograft mice models via inhibition of cell migration and invasion, cell cycle arrest and induction of mitochondrial-mediated apoptosis (Retracted article. See vol. 25, 2023)

被引：33
作者：
Du, Guang-Yu [1 ]
He, Sheng-Wei [1 ]
Zhang, Lu [1 ]
Sun, Chuan-Xiu [1 ]
Mi, Li-Dong [1 ]
Sun, Zue-Gang [1 ]
机构：
[1] Dalian Med Univ, Affiliated Hosp 2, Dept Bone Surg, 467 Zhongshan Rd, Dalian 116023, Liaoning, Peoples R China
来源：
ONCOLOGY LETTERS | 2018年 / 16卷 / 05期
关键词：
apoptosis; osteosarcoma; hesperidin; flow cytometry; cell cycle; CANCER; THERAPY; CHEMOTHERAPY; FLAVONOIDS;
D O I：
10.3892/ol.2018.9439
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The objective of the present study was to investigate the anticancer properties of hesperidin against human osteosarcoma MG-63 cells. Its effects on apoptosis, cell migration, cell invasion and cell cycle arrest, and its effects on tumor volume and weight were also evaluated in the present study. MTS assay was used to study the cytotoxic effects of the compound on cell viability. Effects on apoptosis and cell cycle arrest were evaluated by flow cytometry. In vitro wound healing assay and Matrigel assay were performed to study the effects of hesperidin on cell migration and cell invasion, respectively. Hesperidin exerted dose-dependent and time-dependent growth inhibitory effects on cervical cancer cells with IC50 values of 33.5, 23.8 and 17.6 mu M, respectively, at 24, 48 and 72 h time intervals. Hesperidin led to early and late apoptosis induction in these cells. Hesperidin-treated cells also led to G2/M phase cell cycle arrest, which exhibited strong dose-dependence. Hesperidin treatment also led to inhibition of cell migration and invasion.
引用
收藏
页码：6299 / 6306
页数：8
相关论文
共 22 条
[11]  
Guo W, 1999, CHINESE MED J-PEKING, V112, P1101
[12]   Naturally occurring coumarins inhibit human cytochromes P450 and block benzo[a]pyrene and 7,12-dimethylbenz[a]anthracene DNA adduct formation in MCF-7 cells [J].
Kleiner, HE ;
Reed, MJ ;
DiGiovanni, J .
CHEMICAL RESEARCH IN TOXICOLOGY, 2003, 16 (03) :415-422
[13]   B7-H4 expression in renal cell carcinoma and tumor vasculature: Associations with cancer progression and survival [J].
Krambeck, Amy E. ;
Thompson, R. Houston ;
Dong, Haidong ;
Lohse, Christine M. ;
Park, Eugene S. ;
Kuntz, Susan M. ;
Leibovich, Bradley C. ;
Blute, Michael L. ;
Cheville, John C. ;
Kwon, Eugene D. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (27) :10391-10396
[14]   Primary bone osteosarcoma in the pediatric age: State of the art [J].
Longhi, Alessandra ;
Errani, Costantino ;
De Paolis, Massimiliano ;
Mercuri, Mario ;
Bacci, Gaetano .
CANCER TREATMENT REVIEWS, 2006, 32 (06) :423-436
[15]   Osteosarcoma treatment - Where do we stand? A state of the art review [J].
Luetke, Anja ;
Meyers, Paul A. ;
Lewis, Ian ;
Juergens, Heribert .
CANCER TREATMENT REVIEWS, 2014, 40 (04) :523-532
[16]   Natural products in cancer chemotherapy: past, present and future [J].
Mann, J .
NATURE REVIEWS CANCER, 2002, 2 (02) :143-148
[17]   Biology and therapeutic advances for pediatric osteosarcoma [J].
Marina, N ;
Gebhardt, M ;
Teot, L ;
Gorlick, R .
ONCOLOGIST, 2004, 9 (04) :422-441
[18]  
Middleton E, 2000, PHARMACOL REV, V52, P673
[19]   Breast cancer cell survival signal is affected by bergapten combined with an ultraviolet irradiation [J].
Panno, Maria Luisa ;
Giordano, Francesca ;
Mastroianni, Fabrizia ;
Palma, M. Grazia ;
Bartella, Viviana ;
Carpino, Amalia ;
Aquila, Saveria ;
Ando, Sebastiano .
FEBS LETTERS, 2010, 584 (11) :2321-2326
[20]   4-Hydroxycoumarin disorganizes the actin cytoskeleton in B16-F10 melanoma cells but not in B82 fibroblasts, decreasing their adhesion to extracellular matrix proteins and motility [J].
Velasco-Velázquez, MA ;
Agramonte-Hevia, J ;
Barrera, D ;
Jiménez-Orozco, A ;
García-Mondragón, MJ ;
Mendoza-Patiño, N ;
Landa, A ;
Mandoki, J .
CANCER LETTERS, 2003, 198 (02) :179-186
123