Selenium speciation in rat colon tissues

被引：2

作者：

Roman, Marco ^{[1
]}

Fernandez Sanchez, Maria Luisa ^{[2
]}

Sanz-Medel, Alfredo ^{[2
]}

Gonzalez Iglesias, Hector ^{[2
]}

Cescon, Paolo ^{[1
,3
]}

Barbante, Carlo ^{[1
,3
]}

机构：

[1] Univ Ca Foscari Venice, Dept Environm Sci, I-30123 Venice, Italy

[2] Univ Oviedo, Dept Phys & Analyt Chem, E-33006 Oviedo, Spain

[3] Inst Dynam Environm Proc IDPA CNR, I-30123 Venice, Italy

来源：

JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY | 2011年 / 26卷 / 01期

关键词：

PERFORMANCE LIQUID-CHROMATOGRAPHY; HPLC-ICP-MS; PLASMA-MASS SPECTROMETRY; ENZYMATIC PROBE SONICATION; SAMPLE PREPARATION; SELENOAMINO ACIDS; SE-SPECIATION; EXTRACTION; CANCER; SELENOPROTEINS;

D O I：

10.1039/c0ja00116c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A study of selenium (Se) speciation in rat colon tissues is presented. Four different procedures for the extraction of Se compounds were evaluated in terms of recovery and species preservation. Total Se in tissue and extracts was determined by ICP-MS and isotope dilution analysis. The selected and optimized protocol allowed an extraction of 43% of Se, while continuously bubbling nitrogen in the solution during the procedure was mandatory to prevent the oxidative degradation of selenoproteins. Speciation analysis was then performed on the extracts using size exclusion-and anion exchange-HPLC for species separation. A number of Se compounds were detected in rat colon extracts, and individually quantified by coupling HPLC-ICP-MS and species-unspecific on-line (post-column) isotope dilution analysis. Among the isolated selenospecies, the two major proteins glutathione peroxidase type 2 and thioredoxin reductase type 1 have been potentially identified by their molecular weight using MALDI-TOF-MS.

引用

页码：100 / 108

页数：9

共 44 条

[1] Transcript Analysis of the Selenoproteome Indicates That Dietary Selenium Requirements of Rats Based on Selenium-Regulated Selenoprotein mRNA Levels Are Uniformly Less Than Those Based on Glutathione Peroxidase Activity [J].