Species Difference of CD137 Ligand Signaling in Human and Murine Monocytes

被引:19
作者
Tang, Qianqiao [1 ,2 ,3 ]
Jiang, Dongsheng [1 ,3 ]
Shao, Zhe [1 ,3 ]
Gomez, Julia M. Martinez [1 ,3 ]
Schwarz, Herbert [1 ,2 ,3 ]
机构
[1] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Physiol, Singapore 117595, Singapore
[2] Natl Univ Singapore, NUS Grad Sch Integrat Sci & Engn, Singapore 117595, Singapore
[3] Natl Univ Singapore, Yong Loo Lin Sch Med, Immunol Programme, Singapore 117595, Singapore
来源
PLOS ONE | 2011年 / 6卷 / 01期
关键词
HUMAN DENDRITIC CELLS; RECEPTOR FAMILY; HUMAN; 4-1BB; PROLIFERATION; DIFFERENTIATION; MEMBER; IDENTIFICATION; IMMUNOTHERAPY; ACTIVATION; EXPRESSION;
D O I
10.1371/journal.pone.0016129
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Stimulation of CD137 ligand on human monocytes has been shown to induce DC differentiation, and these CD137L-DCs are more potent than classical DCs, in stimulating T cell responses in vitro. To allow an in vivo evaluation of the potency of CD137L-DCs in murine models we aimed at generating murine CD137L-DCs. Methodology/Principal Findings: When stimulated through CD137 ligand murine monocytes responded just as human monocytes with an increased adherence, morphological changes, proliferation and an increase in viable cell numbers. But CD137 ligand signaling did not induce expression of inflammatory cytokines and costimulatory molecules in murine monocytes and these cells had no T cell stimulatory activity. Murine monocytes did not differentiate to inflammatory DCs upon CD137 ligand signaling. Furthermore, while CD137 ligand signaling induces maturation of human immature classical DCs it failed to do so with murine immature classical DCs. Conclusions/Significance: These data demonstrate that both human and murine monocytes become activated by CD137 ligand signaling but only human and not murine monocytes differentiate to inflammatory DCs.
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页数:8
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