The apicomplexan Cryptosporidium parvum possesses a single mitochondrial-type ferredoxin and ferredoxin:NADP+ reductase system

被引：4

作者：

Lei, Cheng ^{[1
,2
]}

Rider, S. Dean, Jr. ^{[3
]}

Wang, Cai ^{[1
,2
]}

Zhang, Haili ^{[3
]}

Tan, Xiangshi ^{[1
,2
,3
]}

Zhu, Guan ^{[3
,4
]}

机构：

[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

[2] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China

[3] Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Pathobiol, College Stn, TX 77840 USA

[4] Texas A&M Univ, Fac Genet Program, College Stn, TX 77840 USA

来源：

PROTEIN SCIENCE | 2010年 / 19卷 / 11期

基金：

中国国家自然科学基金; 美国国家卫生研究院;

关键词：

apicomplexan; Cryptosporidium; ferredoxin; ferredoxin:NADP(+) reductase; electron transfer; HUMAN MALARIA PARASITE; FATTY-ACID SYNTHESIS; TOXOPLASMA-GONDII; ESCHERICHIA-COLI; IN-VITRO; PLASMODIUM-FALCIPARUM; EIMERIA-TENELLA; GENOME-SEQUENCE; APICOPLAST; OXIDOREDUCTASE;

D O I：

10.1002/pro.487

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have successfully expressed recombinant mitochondrial-type ferredoxin (mtFd) and ferredoxin:NADP(+) reductase (mtFNR) from Ctyptosporidium parvum and characterized their biochemical features for the first time for an apicomplexan. Both C. parvum mtFd (CpmtFd) and FNR (CpmtFNR) were obtained and purified as holo-proteins, in which the correct assembly of [2Fe-2S] cluster in Fd and that of FAD in FNR were confirmed and characterized by UV/vis and electron paramagnetic resonance. These proteins were fully functional and CpmtFNR was capable of transferring electrons from NADPH to CpmtFd in a cytochrome c-coupled assay that followed a typical Michaelis-Menten kinetics. Apicomplexan mtFd and mtFNR proteins were evolutionarily divergent from their counterparts in humans and animals and could be explored as potential drug targets in Cryptosporidium and other apicomplexans.

引用

页码：2073 / 2084

页数：12

共 40 条

[1] Complete genome sequence of the apicomplexan, Cryptosporidium parvum [J].

Abrahamsen, MS ;

Templeton, TJ ;

Enomoto, S ;

Abrahante, JE ;

Zhu, G ;

Lancto, CA ;

Deng, MQ ;

Liu, C ;

Widmer, G ;

Tzipori, S ;

Buck, GA ;

Xu, P ;

Bankier, AT ;

Dear, PH ;

Konfortov, BA ;

Spriggs, HF ;

Iyer, L ;

Anantharaman, V ;

Aravind, L ;

Kapur, V .

SCIENCE, 2004, 304 (5669) :441-445

[2] Structural and functional diversity of ferredoxin-NADP+ reductases [J].

Aliverti, Alessandro ;

Pandini, Vittorio ;

Pennati, Andrea ;

de Rosa, Matteo ;

Zanetti, Giuliana .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2008, 474 (02) :283-291

[3] Soluble expression and purification of the oxidoreductase component of toluene 4-monooxygenase [J].

Bailey, Lucas J. ;

Elsen, Nathaniel L. ;

Pierce, Brad S. ;

Fox, Brian G. .

PROTEIN EXPRESSION AND PURIFICATION, 2008, 57 (01) :9-16

[4] The ferredoxin-NADP+ reductase/ferredoxin electron transfer system of Plasmodium falciparum [J].

Balconi, Emanuela ;

Pennati, Andrea ;

Crobu, Danila ;

Pandini, Vittorio ;

Cerutti, Raffaele ;

Zanetti, Giuliana ;

Aliverti, Alessandro .

FEBS JOURNAL, 2009, 276 (14) :4249-4260

[5] Structure of Synechococcus elongatus [Fe2S2] ferredoxin in solution [J].

Baumann, B ;

Sticht, H ;

Scharpf, M ;

Sutter, M ;

Haehnel, W ;

Rosch, P .

BIOCHEMISTRY, 1996, 35 (39) :12831-12841

[6] Toxoplasma gondii:: analysis of the active site insertion of its ferredoxin-NADP+-reductase by peptide-specific antibodies and homology-based modeling [J].

Bednarek, A ;

Wiek, S ;

Lingelbach, K ;

Seeber, F .

EXPERIMENTAL PARASITOLOGY, 2003, 103 (1-2) :68-77

[7]

BRANDT ME, 1993, J BIOL CHEM, V268, P17126

[8] Biochemical characterization of enoyl reductase involved in Type II fatty acid synthesis in the intestinal coccidium Eimeria tenella (Phylum Apicomplexa) [J].

Cai, Xiaomin ;

Fuller, A. Lorraine ;

McDougald, Larry R. ;

Tan, Xiangshi ;

Cai, Jianping ;

Wang, Feng ;

Sacchettini, James C. ;

Zhu, Guan .

FEMS MICROBIOLOGY LETTERS, 2007, 272 (02) :238-244

[9] Functional characterization of an evolutionarily distinct phosphopantetheinyl transferase in the apicomplexan Cryptosporidium parvum [J].

Cai, XM ;

Herschap, D ;

Zhu, G .

EUKARYOTIC CELL, 2005, 4 (07) :1211-1220

[10] Intron-containing β-tubulin transcripts in Cryptosporidium parvum cultured in vitro [J].

Cai, XM ;

Lancto, CA ;

Abrahamsen, MS ;

Zhu, G .

MICROBIOLOGY-SGM, 2004, 150 :1191-1195

← 1 2 3 4 →