Facile solid-phase synthesis of an antifreeze glycoprotein

被引：0

作者：

Tseng, PH ^{[1
]}

Jiaang, WT ^{[1
]}

Chang, MY ^{[1
]}

Chen, ST ^{[1
]}

机构：

[1] Acad Sinica, Inst Biol Chem, Taipei 115, Taiwan

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2001年 / 7卷 / 03期

关键词：

antifreeze; glycoproteins; peptides; solid-phase synthesis; T-F antigen;

D O I：

10.1002/1521-3765(20010202)7:3<585::AID-CHEM585>3.0.CO;2-6

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The antifreeze glycoproteins (AFGPs) 1 are composed of a repeating tripeptide unit (Ala-Thr-Ala) in which the threonine residue is glycosylated with the disaccharide beta -D-Gal-(1-->3)-alpha -D-GalNAc. A new procedure for synthesizing AFGPs using Fmoc-(Ac-4-beta -D-Gal-(1-->3)-benzylidene-alpha -D-GalNAc)Thr-OH (10) as a building block has been developed. Total synthesis of the AFGPs (n = 4, 8) in overall yields of 61 % and 33 %, respectively, has demonstrated the usefulness of the method. The synthetic AFGPs 1 (n = 4, 8) showed a similar conformation to the native AFGPs in their circular dichroism spectra.

引用

页码：585 / 590

页数：6

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