Effects of Wi-Fi Radiofrequency Radiation on Carbapenem-Resistant Klebsiella pneumoniae

被引:1
作者
Said-Salman, Ilham [1 ,2 ]
Yassine, Wissam [1 ]
Rammal, Ali [3 ]
Hneino, Mohammad [4 ]
Yusef, Hoda [5 ]
Moustafa, Mohamed [6 ]
机构
[1] Lebanese Univ, Fac Sci, Dept Biochem, Hadath, Lebanon
[2] Beirut Arab Univ, Fac Sci, Dept Biol Sci, Debiyeh, Lebanon
[3] Univ St Joseph, Dept Med, Fac Med, Beirut, Lebanon
[4] Lebanese Univ, Fac Hlth, Dept Lab Sci, Hadath, Lebanon
[5] Alexandria Univ, Fac Sci, Dept Bot & Microbiol, Alexandria, Egypt
[6] Alexandria Univ, Fac Sci, Dept Biochem, Alexandria, Egypt
关键词
electromagnetic fields; wireless fidelity; biofilm; transmission electron microscopy; Klebsiella pneumonia; BIOFILM FORMATION; GROWTH-RATE; ANTIBIOTIC-SENSITIVITY; ELECTROMAGNETIC-FIELDS; STAPHYLOCOCCUS-AUREUS; ESCHERICHIA-COLI; IRRADIATION;
D O I
10.1002/bem.22364
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The hazardous consequences of electromagnetic field (EMF) exposure represent a public health concern. Common sources of EMF include smartphones and wireless fidelity (Wi-Fi). The aim of our study is to assess whether exposure to Wi-Fi radiofrequency radiation influences the pathogenic traits of carbapenem-resistant Klebsiella pneumoniae. The susceptibility to antibiotics was evaluated by the determination of minimum inhibitory concentrations (MIC). In this study, K. pneumoniae showed a non-linear response to treatments with Colistin and Gentamycin following different Wi-Fi exposure periods. Transmission electron microscopy revealed morphological changes in the bacterial cell membrane within 24 h of Wi-Fi exposure. Crystal violet quantification and quantitative real-time polymerase chain reaction showed that the ability to form biofilms was greater in Wi-Fi exposed K. pnemoniae when compared to control. Moreover, higher levels of bcsA, mrkA, and luxS messenger RNAs were observed. Our data suggest that Wi-Fi exposure can influence bacteria in a stressful way, leading to an alteration in their antibiotic susceptibility, morphological changes, and cumulative biofilm formation. (c) 2021 Bioelectromagnetics Society.
引用
收藏
页码:575 / 582
页数:8
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