Enhanced thermostability of halo-tolerant glutaminase from Bacillus licheniformis ATCC 14580 by immobilization onto nano magnetic cellulose sheet and its application in production of glutamic acid

被引：8
作者：
Baskaran, Ram [1 ]
Bandikari, Ramesh [1 ]
Zuo, Wu [2 ]
Qian, Jiaxin [1 ]
Liu, Ziduo [1 ]
机构：
[1] Huazhong Agr Univ, State Key Lab Agr Microbiol, Coll Life Sci & Technol, Wuhan 430070, Hubei, Peoples R China
[2] Chinese Acad Sci, Univ Chinese Acad Sci, Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, Beijing, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 119卷
基金：
中国国家自然科学基金;
关键词：
Glutaminase; Bacillus licheniformis; Thermostability; Immobilization; Glutamic acid; CANDIDA-RUGOSA; ENZYMATIC DEAMIDATION; PROTEIN-GLUTAMINASE; MESOPOROUS SILICA; SOY PROTEIN; PURIFICATION; LIPASE; ADAPTATION; EXPRESSION; SUPPORTS;
D O I：
10.1016/j.ijbiomac.2018.08.030
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A halo-tolerant glutaminase gene (BlglsA) was isolated from Bacillus licheniformis. Heterologous expression of BlglsA revealed that it encodes for a 36 kDa protein containing 327 amino acid residues. The purified enzyme showed optimal activity at a pH of 9.5 while 35 degrees C was found to be the optimum temperature. The enzyme retained about 92 and 97% stability at pH 12 and temperature (40 degrees C) respectively. Subsequent immobilization of BIgIsA on nano magnetic cellulose sheet (NMCS) led to an enhanced tolerance to higher temperature. NMCS-BlglsA showed optimum activity at 45 degrees C, although it was stable even at 60 degrees C. NaCI tolerance (>= 90% in 0.3 M) was almost similar to BIgIsA and NMCS-BIgIsA. The metal ions Fe2+ (5 mM) and Mn2+ (2.5 mM) improved the BIgIsA relative activity by 61 and 48%, respectively. In contrast, 5 mM Mn2+ was found suitable to enhance the activity of NMCS-BlglsA up to 72%. The production of glutamic acid by NMCS-BIgIsA was 1.61 g/l in 48 h. Reusability test of NMCS-BIgIsA showed 76 and 35% retention of the actual activity after 4th and 7th cycle, respectively. Such remarkable biochemical properties of NMCS-BIgIsA make it an attractive enzyme for food industries. (C) 2018 Elsevier B.V. All rights reserved.
引用
收藏
页码：1256 / 1263
页数：8
相关论文
共 54 条
[1]   IMMOBILIZATION OF ASPERGILLUS-NIGER NRC-107 XYLANASE AND BETA-XYLOSIDASE, AND PROPERTIES OF THE IMMOBILIZED ENZYMES [J].
ABDELNABY, MA .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1993, 38 (1-2) :69-81
[2]   Characterization of microcrystalline cellulose prepared from lignocellulosic materials. Part I. Acid catalyzed hydrolysis [J].
Adel, Abeer M. ;
El -Wahab, Zeinab H. Abd ;
Ibrahim, Atef A. ;
Al-Shemy, Mona T. .
BIORESOURCE TECHNOLOGY, 2010, 101 (12) :4446-4455
[3]   IMMOBILIZATION OF CELLULASES ON AMINE AND ALDEHYDE FUNCTIONALIZED Fe2O3 MAGNETIC NANOPARTICLES [J].
Alahakoon, Thilini ;
Koh, Jun Wei ;
Chong, Xiang Wen Cindy ;
Lim, Wallace Tse Loong .
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 2012, 42 (03) :234-248
[4]   Bio-affinity mediated immobilization of lipase onto magnetic cellulose nanospheres for high yield biodiesel in one time addition of methanol [J].
Bandikari, Ramesh ;
Qian, Jiaxin ;
Baskaran, Ram ;
Liu, Ziduo ;
Wu, Gaobing .
BIORESOURCE TECHNOLOGY, 2018, 249 :354-360
[5]   Purification and characterization of extracellular glutaminase from Aspergillus oryzae NRRL 32567 [J].
Bazaraa, Wael ;
Alian, Ahmed ;
El-Shimi, Nagwa ;
Mohamed, Reda .
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2016, 6 :76-81
[6]   Recent developments in L-glutaminase production and applications - An overview [J].
Binod, Parameswaran ;
Sindhu, Raveendran ;
Madhavan, Aravind ;
Abraham, Amith ;
Mathew, Anil Kuruvilla ;
Beevi, Ummalyma Sabeela ;
Sukumaran, Rajeev K. ;
Singh, Sudhir P. ;
Pandey, Ashok .
BIORESOURCE TECHNOLOGY, 2017, 245 :1766-1774
[7]   Functionalization of mesoporous silica for lipase immobilization -: Characterization of the support and the catalysts [J].
Blanco, RM ;
Terreros, P ;
Fernández-Pérez, M ;
Otero, C ;
Díaz-González, G .
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 2004, 30 (02) :83-93
[8]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[9]   Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis [J].
Brown, Greg ;
Singer, Alex ;
Proudfoot, Michael ;
Skarina, Tatiana ;
Kim, Youngchang ;
Chang, Changsoo ;
Dementieva, Irina ;
Kuznetsova, Ekaterina ;
Gonzalez, Claudio F. ;
Joachimiak, Andrzej ;
Savchenko, Alexei ;
Yakunin, Alexander F. .
BIOCHEMISTRY, 2008, 47 (21) :5724-5735
[10]   Immobilization of catalase into chemically crosslinked chitosan beads [J].
Çetinus, SA ;
Öztop, HN .
ENZYME AND MICROBIAL TECHNOLOGY, 2003, 32 (07) :889-894
123456