Fungal pathogen protection in potato by expression of a plant defensin peptide

被引：341

作者：

Gao, AG ^{[1
]}

Hakimi, SM ^{[1
]}

Mittanck, CA ^{[1
]}

Wu, Y ^{[1
]}

Woerner, BM ^{[1
]}

Stark, DM ^{[1
]}

Shah, DM ^{[1
]}

Liang, JH ^{[1
]}

Rommens, CMT ^{[1
]}

机构：

[1] Monsanto Co, St Louis, MO 63198 USA

来源：

NATURE BIOTECHNOLOGY | 2000年 / 18卷 / 12期

关键词：

potato; defensins; transgenic; disease control; Verticillium;

D O I：

10.1038/82436

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Defensins are small cysteine-rich peptides with antimicrobial activity. We demonstrate that the alfalfa antifungal peptide (alfAFP) defensin isolated from seeds of Medicago sativa displays strong activity against the agronomically important fungal pathogen Verticillium dahliae, Expression of the alfAFP peptide in transgenic potato plants provides robust resistance in the greenhouse, Importantly, this resistance is maintained under field conditions. There have been no previous demonstrations of a single transgene imparting a disease resistance phenotype that is at least equivalent to those achieved through current practices using fumigants.

引用

页码：1307 / 1310

页数：4

共 20 条

[1]

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[2]

BARRY G, 1992, J CELL BIOCH SF, V16, P202

[3]

Broekaert WF, 1997, CRIT REV PLANT SCI, V16, P297, DOI 10.1080/713608148

[4]

CORNELISSEN BJC, 1997, Patent No. 5670706