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A mitofusin-dependent docking ring complex triggers mitochondrial fusion in vitro
被引:89
作者:

Brandt, Tobias
论文数: 0 引用数: 0
h-index: 0
机构:
Max Planck Inst Biophys, Frankfurt, Germany Max Planck Inst Biophys, Frankfurt, Germany

Cavellini, Laetitia
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h-index: 0
机构:
Univ Paris 04, Inst Biol Physicochim, Lab Biol Mol & Cellulaire Eucaryotes, Paris, France Max Planck Inst Biophys, Frankfurt, Germany

Kuehlbrandt, Werner
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机构:
Max Planck Inst Biophys, Frankfurt, Germany Max Planck Inst Biophys, Frankfurt, Germany

Cohen, Mickael M.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Paris 04, Inst Biol Physicochim, Lab Biol Mol & Cellulaire Eucaryotes, Paris, France Max Planck Inst Biophys, Frankfurt, Germany
机构:
[1] Max Planck Inst Biophys, Frankfurt, Germany
[2] Univ Paris 04, Inst Biol Physicochim, Lab Biol Mol & Cellulaire Eucaryotes, Paris, France
来源:
关键词:
INNER-MEMBRANE-FUSION;
DYNAMIN-LIKE PROTEIN;
SACCHAROMYCES-CEREVISIAE;
STRUCTURAL BASIS;
REQUIRES;
YEAST;
ATLASTIN;
GTP;
DOMAIN;
OUTER;
D O I:
10.7554/eLife.14618
中图分类号:
Q [生物科学];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
Fusion of mitochondrial outer membranes is crucial for proper organelle function and involves large GTPases called mitofusins. The discrete steps that allow mitochondria to attach to one another and merge their outer membranes are unknown. By combining an in vitro mitochondrial fusion assay with electron cryo-tomography (cryo-ET), we visualize the junction between attached mitochondria isolated from Saccharomyces cerevisiae and observe complexes that mediate this attachment. We find that cycles of GTP hydrolysis induce progressive formation of a docking ring structure around extended areas of contact. Further GTP hydrolysis triggers local outer membrane fusion at the periphery of the contact region. These findings unravel key features of mitofusin-dependent fusion of outer membranes and constitute an important advance in our understanding of how mitochondria connect and merge.
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[1]
Membrane Tethering and Nucleotide-dependent Conformational Changes Drive Mitochondrial Genome Maintenance (Mgm1) Protein-mediated Membrane Fusion
[J].
Abutbul-Ionita, Inbal
;
Rujiviphat, Jarungjit
;
Nir, Iftach
;
McQuibban, G. Angus
;
Danino, Dganit
.
JOURNAL OF BIOLOGICAL CHEMISTRY,
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:36634-36638

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[2]
A Mutation Associated with CMT2A Neuropathy Causes Defects in Fzo1 GTP Hydrolysis, Ubiquitylation, and Protein Turnover
[J].
Amiott, Elizabeth A.
;
Cohen, Mickael M.
;
Saint-Georges, Yann
;
Weissman, Allan M.
;
Shaw, Janet M.
.
MOLECULAR BIOLOGY OF THE CELL,
2009, 20 (23)
:5026-5035

Amiott, Elizabeth A.
论文数: 0 引用数: 0
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[3]
Ugo1 and Mdm30 act sequentially during Fzo1-mediated mitochondrial outer membrane fusion
[J].
Anton, Fabian
;
Fres, Julia M.
;
Schauss, Astrid
;
Pinson, Benoit
;
Praefcke, Gerrit J. K.
;
Langer, Thomas
;
Escobar-Henriques, Mafalda
.
JOURNAL OF CELL SCIENCE,
2011, 124 (07)
:1126-1135

Anton, Fabian
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h-index: 0
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Univ Cologne, Inst Genet, D-50674 Cologne, Germany
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Univ Cologne, Inst Genet, D-50674 Cologne, Germany
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Schauss, Astrid
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机构:

论文数: 引用数:
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机构:
[4]
A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation
[J].
Beckett, D
;
Kovaleva, E
;
Schatz, PJ
.
PROTEIN SCIENCE,
1999, 8 (04)
:921-929

Beckett, D
论文数: 0 引用数: 0
h-index: 0
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Kovaleva, E
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h-index: 0
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Schatz, PJ
论文数: 0 引用数: 0
h-index: 0
机构: Univ Maryland Baltimore Cty, Dept Chem & Biochem, Baltimore, MD 21250 USA
[5]
Structures of the atlastin GTPase provide insight into homotypic fusion of endoplasmic reticulum membranes
[J].
Bian, Xin
;
Klemm, Robin W.
;
Liu, Tina Y.
;
Zhang, Miao
;
Sun, Sha
;
Sui, Xuewu
;
Liu, Xinqi
;
Rapoport, Tom A.
;
Hu, Junjie
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
2011, 108 (10)
:3976-3981

Bian, Xin
论文数: 0 引用数: 0
h-index: 0
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Nankai Univ, Tianjin Key Lab Prot Sci, Tianjin 300071, Peoples R China
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Klemm, Robin W.
论文数: 0 引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Howard Hughes Med Inst, Dept Cell Biol, Boston, MA 02115 USA Nankai Univ, Tianjin Key Lab Prot Sci, Tianjin 300071, Peoples R China

Liu, Tina Y.
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h-index: 0
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Zhang, Miao
论文数: 0 引用数: 0
h-index: 0
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Nankai Univ, Tianjin Key Lab Prot Sci, Tianjin 300071, Peoples R China
Nankai Univ, Dept Genet & Cell Biol, Coll Life Sci, Tianjin 300071, Peoples R China Nankai Univ, Tianjin Key Lab Prot Sci, Tianjin 300071, Peoples R China

Sun, Sha
论文数: 0 引用数: 0
h-index: 0
机构:
Nankai Univ, Tianjin Key Lab Prot Sci, Tianjin 300071, Peoples R China
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Liu, Xinqi
论文数: 0 引用数: 0
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Nankai Univ, Tianjin Key Lab Prot Sci, Tianjin 300071, Peoples R China
Nankai Univ, Dept Biochem & Mol Biol, Coll Life Sci, Tianjin 300071, Peoples R China Nankai Univ, Tianjin Key Lab Prot Sci, Tianjin 300071, Peoples R China

Rapoport, Tom A.
论文数: 0 引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Howard Hughes Med Inst, Dept Cell Biol, Boston, MA 02115 USA Nankai Univ, Tianjin Key Lab Prot Sci, Tianjin 300071, Peoples R China

Hu, Junjie
论文数: 0 引用数: 0
h-index: 0
机构:
Nankai Univ, Tianjin Key Lab Prot Sci, Tianjin 300071, Peoples R China
Nankai Univ, Dept Genet & Cell Biol, Coll Life Sci, Tianjin 300071, Peoples R China Nankai Univ, Tianjin Key Lab Prot Sci, Tianjin 300071, Peoples R China
[6]
Dynamin Assembly Strategies and Adaptor Review Proteins in Mitochondrial Fission
[J].
Bui, Huyen T.
;
Shaw, Janet M.
.
CURRENT BIOLOGY,
2013, 23 (19)
:R891-R899

Bui, Huyen T.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Utah, Sch Med, Dept Biochem, Salt Lake City, UT 84112 USA Univ Utah, Sch Med, Dept Biochem, Salt Lake City, UT 84112 USA

Shaw, Janet M.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Utah, Sch Med, Dept Biochem, Salt Lake City, UT 84112 USA Univ Utah, Sch Med, Dept Biochem, Salt Lake City, UT 84112 USA
[7]
Structural basis for conformational switching and GTP loading of the large G protein atlastin
[J].
Byrnes, Laura J.
;
Singh, Avtar
;
Szeto, Kylan
;
Benvin, Nicole M.
;
O'Donnell, John P.
;
Zipfel, Warren R.
;
Sondermann, Holger
.
EMBO JOURNAL,
2013, 32 (03)
:369-384

Byrnes, Laura J.
论文数: 0 引用数: 0
h-index: 0
机构:
Cornell Univ, Coll Vet Med, Dept Mol Med, Ithaca, NY 14853 USA Cornell Univ, Coll Vet Med, Dept Mol Med, Ithaca, NY 14853 USA

Singh, Avtar
论文数: 0 引用数: 0
h-index: 0
机构:
Cornell Univ, Dept Biomed Engn, Ithaca, NY 14853 USA Cornell Univ, Coll Vet Med, Dept Mol Med, Ithaca, NY 14853 USA

Szeto, Kylan
论文数: 0 引用数: 0
h-index: 0
机构:
Cornell Univ, Dept Appl & Engn Phys, Ithaca, NY 14853 USA Cornell Univ, Coll Vet Med, Dept Mol Med, Ithaca, NY 14853 USA

Benvin, Nicole M.
论文数: 0 引用数: 0
h-index: 0
机构: Cornell Univ, Coll Vet Med, Dept Mol Med, Ithaca, NY 14853 USA

O'Donnell, John P.
论文数: 0 引用数: 0
h-index: 0
机构:
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Zipfel, Warren R.
论文数: 0 引用数: 0
h-index: 0
机构:
Cornell Univ, Dept Biomed Engn, Ithaca, NY 14853 USA Cornell Univ, Coll Vet Med, Dept Mol Med, Ithaca, NY 14853 USA

Sondermann, Holger
论文数: 0 引用数: 0
h-index: 0
机构:
Cornell Univ, Coll Vet Med, Dept Mol Med, Ithaca, NY 14853 USA Cornell Univ, Coll Vet Med, Dept Mol Med, Ithaca, NY 14853 USA
[8]
Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle
[J].
Cai, Huaqing
;
Reinisch, Karin
;
Ferro-Novick, Susan
.
DEVELOPMENTAL CELL,
2007, 12 (05)
:671-682

Cai, Huaqing
论文数: 0 引用数: 0
h-index: 0
机构: Yale Univ, Sch Med, Howard Hughes Med Inst, New Haven, CT 06519 USA

Reinisch, Karin
论文数: 0 引用数: 0
h-index: 0
机构: Yale Univ, Sch Med, Howard Hughes Med Inst, New Haven, CT 06519 USA

Ferro-Novick, Susan
论文数: 0 引用数: 0
h-index: 0
机构: Yale Univ, Sch Med, Howard Hughes Med Inst, New Haven, CT 06519 USA
[9]
Sequential requirements for the GTPase domain of the mitofusin Fzo1 and the ubiquitin ligase SCFMdm30 in mitochondrial outer membrane fusion
[J].
Cohen, Mickael M.
;
Amiott, Elizabeth A.
;
Day, Adam R.
;
Leboucher, Guillaume P.
;
Pryce, Erin N.
;
Glickman, Michael H.
;
McCaffery, J. Michael
;
Shaw, Janet M.
;
Weissman, Allan M.
.
JOURNAL OF CELL SCIENCE,
2011, 124 (09)
:1403-1410

Cohen, Mickael M.
论文数: 0 引用数: 0
h-index: 0
机构:
NCI Frederick, Lab Prot Dynam & Signaling, Ctr Canc Res, Ft Detrick, MD 21702 USA NCI Frederick, Lab Prot Dynam & Signaling, Ctr Canc Res, Ft Detrick, MD 21702 USA

Amiott, Elizabeth A.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Utah, Sch Med, Dept Biochem, Salt Lake City, UT 84112 USA NCI Frederick, Lab Prot Dynam & Signaling, Ctr Canc Res, Ft Detrick, MD 21702 USA

Day, Adam R.
论文数: 0 引用数: 0
h-index: 0
机构:
NCI Frederick, Lab Prot Dynam & Signaling, Ctr Canc Res, Ft Detrick, MD 21702 USA NCI Frederick, Lab Prot Dynam & Signaling, Ctr Canc Res, Ft Detrick, MD 21702 USA

Leboucher, Guillaume P.
论文数: 0 引用数: 0
h-index: 0
机构:
NCI Frederick, Lab Prot Dynam & Signaling, Ctr Canc Res, Ft Detrick, MD 21702 USA
Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel NCI Frederick, Lab Prot Dynam & Signaling, Ctr Canc Res, Ft Detrick, MD 21702 USA

Pryce, Erin N.
论文数: 0 引用数: 0
h-index: 0
机构:
Johns Hopkins Univ, Dept Biol, Integrated Imaging Ctr, Baltimore, MD 21218 USA NCI Frederick, Lab Prot Dynam & Signaling, Ctr Canc Res, Ft Detrick, MD 21702 USA

Glickman, Michael H.
论文数: 0 引用数: 0
h-index: 0
机构:
Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel NCI Frederick, Lab Prot Dynam & Signaling, Ctr Canc Res, Ft Detrick, MD 21702 USA

McCaffery, J. Michael
论文数: 0 引用数: 0
h-index: 0
机构:
Johns Hopkins Univ, Dept Biol, Integrated Imaging Ctr, Baltimore, MD 21218 USA NCI Frederick, Lab Prot Dynam & Signaling, Ctr Canc Res, Ft Detrick, MD 21702 USA

Shaw, Janet M.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Utah, Sch Med, Dept Biochem, Salt Lake City, UT 84112 USA NCI Frederick, Lab Prot Dynam & Signaling, Ctr Canc Res, Ft Detrick, MD 21702 USA

Weissman, Allan M.
论文数: 0 引用数: 0
h-index: 0
机构:
NCI Frederick, Lab Prot Dynam & Signaling, Ctr Canc Res, Ft Detrick, MD 21702 USA NCI Frederick, Lab Prot Dynam & Signaling, Ctr Canc Res, Ft Detrick, MD 21702 USA
[10]
Ubiquitin-proteasome-dependent degradation of a mitofusin, a critical regulator of mitochondrial fusion
[J].
Cohen, Mickael M. J.
;
Leboucher, Guillaume P.
;
Livnat-Levanon, Nurit
;
Glickman, Michael H.
;
Weissman, Allan M.
.
MOLECULAR BIOLOGY OF THE CELL,
2008, 19 (06)
:2457-2464

Cohen, Mickael M. J.
论文数: 0 引用数: 0
h-index: 0
机构:
Natl Canc Ctr, Lab Prot Dynam & Signaling, Ft Detrick, MD 21702 USA Natl Canc Ctr, Lab Prot Dynam & Signaling, Ft Detrick, MD 21702 USA

Leboucher, Guillaume P.
论文数: 0 引用数: 0
h-index: 0
机构:
Natl Canc Ctr, Lab Prot Dynam & Signaling, Ft Detrick, MD 21702 USA
Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel Natl Canc Ctr, Lab Prot Dynam & Signaling, Ft Detrick, MD 21702 USA

Livnat-Levanon, Nurit
论文数: 0 引用数: 0
h-index: 0
机构:
Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel Natl Canc Ctr, Lab Prot Dynam & Signaling, Ft Detrick, MD 21702 USA

Glickman, Michael H.
论文数: 0 引用数: 0
h-index: 0
机构:
Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel Natl Canc Ctr, Lab Prot Dynam & Signaling, Ft Detrick, MD 21702 USA

Weissman, Allan M.
论文数: 0 引用数: 0
h-index: 0
机构:
Natl Canc Ctr, Lab Prot Dynam & Signaling, Ft Detrick, MD 21702 USA Natl Canc Ctr, Lab Prot Dynam & Signaling, Ft Detrick, MD 21702 USA