Yeast telomerase protein Est3 is a novel type of GTPase

被引：1
|
作者：
Shubernetskaya, Olga [1 ]
Logvina, Natalia [1 ]
Sharanov, Yury [1 ]
Zvereva, Maria [1 ]
机构：
[1] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119991, Russia
来源：
BIOCHIMIE | 2011年 / 93卷 / 02期
关键词：
Telomerase; Telomere length maintenance; Est3; Noncanonical GTPase; SACCHAROMYCES-CEREVISIAE TELOMERASE; IN-VITRO; REVERSE-TRANSCRIPTASE; OB-FOLD; DIMERIZATION; REPLICATION; DOMAIN; SUBUNIT; RNA;
D O I：
10.1016/j.biochi.2010.09.013
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Telomerase is a key participant of telomere length maintaining system in the majority of eukaryotes. It synthesizes telomere repeats at the 3'-end of telomere DNA using its own RNA template. The Saccharomyces cerevisiae telomerase complex contains the reverse transcriptase (Est2) and RNA subunits (TLC1), as well as number of additional proteins among which Est3 is known to be essential for telomere length maintenance. We have found that Est3 can hydrolyze GTP in vitro and investigated parameters of this reaction. The data obtained suggest that Est3 may function as unusual GTPase in vivo. (C) 2010 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码：202 / 206
页数：5
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