Real-time mapping of a hydrogen peroxide concentration profile across a polymicrobial bacterial biofilm using scanning electrochemical microscopy

被引：135

作者：

Liu, Xiuhui ^{[1
]}

Ramsey, Matthew M. ^{[2
]}

Chen, Xiaole ^{[1
]}

Koley, Dipankar ^{[1
]}

Whiteley, Marvin ^{[2
]}

Bard, Allen J. ^{[1
]}

机构：

[1] Univ Texas Austin, Dept Chem & Biochem, Ctr Electrochem, Austin, TX 78712 USA

[2] Univ Texas Austin, Sect Mol Genet & Microbiol, Austin, TX 78712 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2011年 / 108卷 / 07期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

real-time (detection); metabolite efflux; local concentration; oral flora; Au UME; STREPTOCOCCUS-SANGUINIS; LIVING CELLS; RESISTANCE; ELECTRODE; AG+;

D O I：

10.1073/pnas.1018391108

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Quantitative detection of hydrogen peroxide in solution above a Streptococcus gordonii (Sg) bacterial biofilm was studied in real time by scanning electrochemical microscopy (SECM). The concentration of hydrogen peroxide was determined to be 0.7 mM to 1.6 mM in the presence of 10 mM glucose over a period of 2 to 8 h. The hydrogen peroxide production measured was higher near the biofilm surface in comparison to Sg grown planktonically. Differential hydrogen peroxide production was observed both by fluorometric as well as by SECM measurements. The interaction between two different species in a bacterial biofilm of Sg and Aggregatibacter actinomycetemcomitans (Aa) in terms of hydrogen peroxide production was also studied by SECM. One-directional y-scan SECM measurements showed the unique spatial mapping of hydrogen peroxide concentration across a mixed species biofilm and revealed that hydrogen peroxide concentration varies greatly dependent upon local species composition.

引用

页码：2668 / 2673

页数：6

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