A deep red ratiometric fluorescent probe for accurate detection of peroxynitrite in mitochondria

被引：28

作者：

Han, Rubing ^{[1
]}

Shu, Wei ^{[2
]}

Kang, Hao ^{[1
]}

Duan, Qingxia ^{[1
]}

Zhang, Xiaoli ^{[1
]}

Liang, Chenlu ^{[1
]}

Gao, Mengxu ^{[1
]}

Xu, Liren ^{[1
]}

Jing, Jing ^{[1
]}

Zhang, Xiaoling ^{[1
]}

机构：

[1] Beijing Inst Technol, Sch Chem & Chem Engn,Beijing Key Lab Photoelect E, Minist Educ,Minist Ind & Informat Technol,Analyt, Key Lab Cluster Sci,Key Lab Med Mol Sci & Pharmac, Beijing 100081, Peoples R China

[2] Shandong Univ Technol, Sch Life Sci & Med, Zibo 255000, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2022年 / 1203卷

基金：

中国国家自然科学基金;

关键词：

Fluorescent probe; Peroxynitrite; Ratio; Deep red; Mitochondria; IN-VIVO; SELECTIVE DETECTION; NITRIC-OXIDE; MODEL;

D O I：

10.1016/j.aca.2022.339652

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Peroxynitrite (ONOO-) is widespread within living organisms and has been implicated in many physiological and pathological processes. Since ONOO- is mainly produced in mitochondria, accurate detection of ONOO- in mitochondria can help us understand its specific mechanism of action in the organism. Rather than single-wavelength emissive mitochondrial probes, ratiometric fluorescent probes with longer emission wavelength, large emission shift, and specific mitochondrial targeting properties are more likely to obtain a more accurate ONOO- content in mitochondria. To further avoid the interference by cytoplasmic ONOO-, we constructed a fluorescent probe MXMP with deep red emission and ratio properties, and it will be forbidden to enter the mitochondria after oxidation. In addition to its excellent selectivity and sensitivity, it shifted its fluorescence emission by up to 130 nm, with a detection limit of 84 nM. (C) 2022 Elsevier B.V. All rights reserved.

引用

页数：8

共 41 条

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