American Foulbrood in the Czech Republic: ERIC II Genotype of Paenibacillus Larvae Is Prevalent

被引:7
作者
Biova, Jana [1 ]
Bzdil, Jaroslav [2 ]
Dostalkova, Silvie [1 ]
Petrivalsky, Marek [1 ]
Brus, Jan [3 ]
Carra, Elena [4 ]
Danihlik, Jiri [2 ]
机构
[1] Palacky Univ Olomouc, Dept Biochem, Fac Sci, Olomouc, Czech Republic
[2] State Vet Inst Olomouc, Olomouc, Czech Republic
[3] Palacky Univ Olomouc, Dept Geoinformat, Fac Sci, Olomouc, Czech Republic
[4] Expt Zooprophylact Inst Lombardy & Emilia Romagna, Brescia, Italy
关键词
ERIC genotype; Epizootology; honey bee(Apis mellifera L.); Europe; Dangerous infectious disease; HONEY-BEE; CAUSATIVE AGENT; SUBSP LARVAE; RESISTANCE;
D O I
10.3389/fvets.2021.698976
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
American foulbrood (AFB) is a dangerous disease of honeybees (Apis mellifera) caused by the spore-forming bacterium Paenibacillus larvae. According to the ERIC (enterobacterial repetitive intergenic consensus) classification, five genotypes are distinguished, i.e., I, II, III, IV, and V, which differ in their virulence and prevalence in colonies. In the Czech Republic, AFB prevalence is monitored by the State Veterinary Administration; however, the occurrence of specific P. larvae genotypes within the country remains unknown. In this study, our aim was to genotype field P. larvae strains collected in the Czech Republic according to the ERIC classification. In total, 102 field isolates from colonies with AFB clinical symptoms were collected from various locations in the Czech Republic, and the PCR genotypization was performed using ERIC primers. We confirmed the presence of both ERIC I and II genotypes, while ERIC III, IV, and V were not detected. The majority of samples (n = 82, 80.4%) were identified as ERIC II, while the ERIC I genotype was confirmed only in 20 samples (19.6%). In contrast to other European countries, the ERIC II genotype is predominant in Czech honeybee colonies. The ERIC I genotype was mostly detected in border regions close to Poland, Slovakia, and Austria.
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页数:5
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