Glycine confers neuroprotection through microRNA-301a/PTEN signaling

被引：22

作者：

Chen, Juan ^{[1
,2
]}

Zhuang, Yang ^{[1
]}

Zhang, Zhi-Feng ^{[1
]}

Wang, Shu ^{[1
]}

Jin, Ping ^{[2
]}

He, Chunjiang ^{[3
]}

Hu, Peng-Chao ^{[1
]}

Wang, Ze-Fen ^{[1
]}

Li, Zhi-Qiang ^{[4
]}

Xia, Guang-Ming ^{[5
]}

Li, Gang ^{[5
]}

Wang, Yuan ^{[1
]}

Wan, Qi ^{[1
,4
]}

机构：

[1] Wuhan Univ, Sch Med, Med Res Inst, Dept Physiol,Sch Basic Med Sci, 185 Donghu St, Wuhan 430071, Peoples R China

[2] Cent Hosp Wuhan, Dept Neurol, Wuhan 430060, Peoples R China

[3] Wuhan Univ, Sch Med, Sch Basic Med Sci, Dept Genet, 185 Donghu St, Wuhan 430071, Peoples R China

[4] Wuhan Univ, Sch Med, Zhongnan Hosp, Dept Neurosurg, 169 Donghu St, Wuhan 430071, Peoples R China

[5] Cent Hosp Huanggang, Dept Neurol, Huanggang 438000, Peoples R China

来源：

MOLECULAR BRAIN | 2016年 / 9卷

关键词：

miR-301a; Glycine; PTEN; Neuroprotection; SPINAL-CORD-INJURY; PTEN INHIBITION; AXON REGENERATION; CYTOPROTECTIVE AGENT; FUNCTIONAL DIVERSITY; MICRORNA BIOGENESIS; RECEPTOR FUNCTION; TUMOR-SUPPRESSOR; NEURONAL INJURY; ISCHEMIC-STROKE;

D O I：

10.1186/s13041-016-0241-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Background: Glycine is known to protect against neuronal death. However, the underlying mechanism remains to be elucidated. The microRNA-301a is involved in both biological and pathological processes. But it is not known whether microRNA-301a has a neuroprotective property. In this study, we aimed to determine whether glycine-induced neuroprotection requires microRNA-301a-dependent signaling. Results: We provided the first evidence that glycine increased the expression of microRNA-301a in cultured rat cortical neurons and protected against cortical neuronal death through up-regulation of microRNA-301a after oxygen-glucose deprivation. MicroRNA-301a directly bound the predicted 3'UTR target sites of PTEN and reduced PTEN expression in cortical neurons. We revealed that PTEN down-regulation by microRNA-301a mediated glycine-induced neuroprotective effect following oxygen-glucose deprivation. Conclusions: Our results suggest that 1) microRNA-301a is neuroprotective in oxygen-glucose deprivation-induced neuronal injury; 2) glycine is an upstream regulator of microRNA-301a; 3) glycine confers neuroprotection through microRNA-301a/PTEN signal pathway.

引用

页数：13

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