Isolation of Purified and Live Foxp3+ Regulatory T Cells using FACS Sorting on Scatter Plot

被引：34

作者：

Zhou, Xiaohui ^{[1
,2
]}

Wang, Julie ^{[1
]}

Shi, Wei ^{[3
]}

Brand, David D. ^{[4
]}

Liu, Zhongmin ^{[2
]}

Fan, Huimin ^{[2
]}

Zheng, Song Guo ^{[1
]}

机构：

[1] Univ So Calif, Dept Med, Div Rheumatol & Immunol, Los Angeles, CA USA

[2] Tongji Univ, Ctr Immunoregulat & Tolerance, Shanghai E Hosp, Sch Med, Shanghai 200092, Peoples R China

[3] Univ So Calif, Childrens Hosp Los Angeles, Dept Surg, Dev Biol Program, Los Angeles, CA USA

[4] Vet Affairs Med Ctr, Res Serv, Memphis, TN USA

来源：

JOURNAL OF MOLECULAR CELL BIOLOGY | 2010年 / 2卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Foxp3; TGF-beta; regulatory T cells; IMMUNOLOGICAL SELF-TOLERANCE; TGF-BETA; CUTTING EDGE; EXPRESSION; INDUCTION; IL-2; TH17; CONVERSION; CD25(+);

D O I：

10.1093/jmcb/mjq007

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

There are no ideal ways to identify and isolate viable and purified Foxp3(+) regulatory T cells so far. Here we developed a novel procedure for the isolation of highly purified Foxp3(+) cells using flow cytometry. This method relies on an identification and sorting of the lymphoblast cell population identified on a scatter plot using flow cytometry. We confirmed that greater than 98% of the cells sorted using this technique expressed Foxp3 and displayed a potent suppressive activity. This method provides a valuable tool for the study of the T regulatory cell biology and their therapeutic manipulation.

引用

页码：164 / 169

页数：6

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