Metastatic growth instructed by neutrophil-derived transferrin

被引:53
作者
Liang, Wei [1 ]
Li, Qin [1 ]
Ferrara, Napoleone [1 ]
机构
[1] Univ Calif San Diego, Moores Canc Ctr, La Jolla, CA 92093 USA
关键词
innate immunity; metastasis; tumor microenvironment; breast cancer; GM-CSF; BREAST-CANCER METASTASIS; TUMOR ANGIOGENESIS; GM-CSF; MYELOID CELLS; LUNG; EXPRESSION; RECEPTOR; SECRETION;
D O I
10.1073/pnas.1811717115
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The tumor-promoting functions of neutrophils have been mainly attributed to induction of tumor angiogenesis or suppression of anticancer immunity. However, a direct impact of neutrophils on tumor cell growth and metastasis remains largely uncharacterized. Here, we coupled a proteomic approach with a functional screen to interrogate the secretome of tumor-associated neutrophils. Surprisingly, the iron-transporting protein transferrin was identified as the major mitogen for tumor cells secreted by neutrophils. Depletion of neutrophils inhibited lung metastasis and transferrin production in the metastatic microenvironment. Deletion of transferrin receptor suppressed growth of lung-colonizing tumor cells. Also, media conditioned by neutrophils isolated from metastatic breast cancer patients stimulated growth of human breast cancer cells, an effect that was largely abolished by transferrin immuno-depletion. We identified GM-CSF, which is produced primarily by tumor cells, as a selective inducer of de novo transferrin synthesis in neutrophils through the Jak/Stat5 beta pathway. GM-CSF neutralization or inhibition of Jak kinases curtailed neutrophil transferrin expression in vitro and in vivo as well as cancer metastasis. Thus, transferrin provides a mechanistic link between neutrophils and metastatic growth owing to the ability of tumor-infiltrating neutrophils to locally deliver this growth-promoting protein in response to GM-CSF stimulation. Our study identifies neutrophil-derived transferrin as a key regulator of metastatic tumor cell growth and a therapeutic target for antimetastatic treatment.
引用
收藏
页码:11060 / 11065
页数:6
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