Molecular cloning, expression, and characterization of E2F transcription factor 4 from Antheraea pernyi

被引：8

作者：

Abbas, M. N. ^{[1
]}

Kausar, S. ^{[1
]}

Sun, Y. -X. ^{[1
]}

Sun, Y. ^{[1
]}

Wang, L. ^{[1
]}

Qian, C. ^{[1
]}

Wei, G. -Q. ^{[1
]}

Zhu, B. -J. ^{[1
]}

Liu, C. -L. ^{[1
]}

机构：

[1] Anhui Agr Univ, Coll Life Sci, Hefei 230036, Anhui, Peoples R China

来源：

BULLETIN OF ENTOMOLOGICAL RESEARCH | 2017年 / 107卷 / 06期

基金：

中国国家自然科学基金;

关键词：

quantitative real-time PCR; silkworm; genome; homology; FAMILY; SILKWORM;

D O I：

10.1017/S0007485317000426

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

The E2F transcription factor family is distributed widely in eukaryotes and has been well studied among mammals. In the present study, the E2F transcription factor 4 (E2F4) gene was isolated from fat bodies of Antheraea pernyi and sequenced. E2F4 comprised a 795 bp open reading frame encoding a deduced amino acid sequence of 264 amino acid residues. The recombinant protein was expressed in Escherichia coli (Transetta DE3), and anti-E2F4 antibodies were prepared. The deduced amino acid sequence displayed significant homology to an E2F4-like protein from Bombyx mori L. Quantitative real-time polymerase chain reaction analysis revealed that E2F4 expression was highest in the integument, followed by the fat body, silk glands, and haemocytes. The expression of E2F4 was upregulated in larvae challenged by bacterial (Escherichia coli, Micrococcus luteus), viral (nuclear polyhedrosis virus), and fungal (Beauveria bassiana) pathogens. These observations indicated that E2F4 is an inducible protein in the immune response of A. pernyi and probably in other insects.

引用

页码：839 / 846

页数：8

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