The properties and contribution of the Corynebacterium glutamicum MscS variant to fine-tuning of osmotic adaptation

被引：58

作者：

Boerngen, Kirsten ^{[1
]}

Battle, Andrew R. ^{[4
]}

Moeker, Nina ^{[1
]}

Morbach, Susanne ^{[1
]}

Marin, Kay ^{[1
]}

Martinac, Boris ^{[2
,3
]}

Kraemer, Reinhard ^{[1
]}

机构：

[1] Univ Cologne, Inst Biochem, D-50674 Cologne, Germany

[2] Victor Chang Cardiac Res Inst, Sydney, NSW 2010, Australia

[3] Univ New S Wales, St Vincents Clin Sch, Sydney, NSW 2052, Australia

[4] Univ Queensland, Dept Physiol & Pharmacol, Sch Biomed Sci, St Lucia, Qld 4072, Australia

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2010年 / 1798卷 / 11期

基金：

澳大利亚研究理事会;

关键词：

Mechanosensitive channel; Corynebacterium; Efflux; Osmoregulation; Uptake; Betaine; MECHANOSENSITIVE ION CHANNELS; ESCHERICHIA-COLI MSCS; BETAINE CARRIER BETP; BIOCHEMICAL-CHARACTERIZATION; IDENTIFICATION; CONDUCTION; PRESSURE; SYSTEM; CELLS;

D O I：

10.1016/j.bbamem.2010.06.022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Based on sequence similarity, the mscCG gene product of Corynebacterium glutamicum belongs to the family of MscS-type mechanosensitive channels. In order to investigate the physiological significance of MscCG in response to osmotic shifts in detail, we studied its properties using both patch-clamp techniques and betaine efflux kinetics. After heterologous expression in an Escherichia coli strain devoid of mechanosensitive channels, in patch-clamp analysis of giant E. coli spheroplasts MscCG showed the typical pressure dependent gating behavior of a stretch-activated channel with a current/voltage dependence indicating a strongly rectifying behavior. Apart from that. MscCG is characterized by significant functional differences with respect to conductance, ion selectivity and desensitation behavior as compared to MscS from E. coli. Deletion and complementation studies in C glutamicum showed a significant contribution of MscCG to betaine efflux in response to hypoosmotic conditions. A detailed analysis of concomitant betaine uptake (by the betaine transporter BetP) and efflux (by MscCG) under hyperosmotic conditions indicates that MscCG may act in osmoregulation in C. glutamicum by fine-tuning the steady state concentration of compatible solutes in the cytoplasm which are accumulated in response to hyperosmotic stress. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：2141 / 2149

页数：9

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