Campylobacter jejuni isolated from poultry meat in Brazil: in silico analysis and genomic features of two strains with different phenotypes of antimicrobial susceptibility

被引：11
作者：
De Fatima Rauber Wurfel, Simone [1 ]
Jorge, Sergio [1 ]
De Oliveira, Natasha Rodrigues [1 ]
Kremer, Frederico Schmitt [1 ]
Sanchez, Christian Domingues [1 ]
Campos, Vinicius Farias [1 ]
Da Silva Pinto, Luciano [1 ]
Da Silva, Wladimir Padilha [2 ]
Dellagostin, Odir Antonio [1 ]
机构：
[1] Universidade Fed Pelotas, Centro Desenvolvimento Tecnol, Nucleo Biotecnol, Pelotas, RS, Brazil
[2] Universidade Fed Pelotas, Faculdade Agronomia Eliseu Maciel, Departamento Ciencia Tecnol Agroindustrial, Pelotas, RS, Brazil
来源：
MOLECULAR BIOLOGY REPORTS | 2020年 / 47卷 / 01期
关键词：
Genomics; Bioinformatics; Megaplasmid; Antimicrobial resistance; Virulence factors; FOOD-BORNE PATHOGEN; EFFLUX PUMP; RESISTANCE; SEQUENCE; MECHANISMS; DIVERSITY; VIRULENCE; ALIGNMENT; SURVIVAL; DATABASE;
D O I：
10.1007/s11033-019-05174-y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Campylobacter jejuni is the most common bacterial cause of foodborne diarrheal disease worldwide and is among the antimicrobial resistant "priority pathogens" that pose greatest threat to public health. The genomes of two C. jejuni isolated from poultry meat sold on the retail market in Southern Brazil phenotypically characterized as multidrug-resistant (CJ100) and susceptible (CJ104) were sequenced and analyzed by bioinformatic tools. The isolates CJ100 and CJ104 showed distinct multilocus sequence types (MLST). Comparative genomic analysis revealed a large number of single nucleotide polymorphisms, rearrangements, and inversions in both genomes, in addition to virulence factors, genomic islands, prophage sequences, and insertion sequences. A circular 103-kilobase megaplasmid carrying virulence factors was identified in the genome of CJ100, in addition to resistance mechanisms to aminoglycosides, beta-lactams, macrolides, quinolones, and tetracyclines. The molecular characterization of distinct phenotypes of foodborne C. jejuni and the discovery of a novel virulence megaplasmid provide useful data for pan-genome and large-scale studies to monitor the virulent C. jejuni in poultry meat is warranted.
引用
收藏
页码：671 / 681
页数：11
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