Electrochemical impedance spectroscopy of polypyrrole based electrochemical immunosensor

被引：186
作者：
Ramanavicius, A. [1 ]
Finkelsteinas, A. [1 ]
Cesiulis, H. [2 ]
Ramanaviciene, A. [1 ,3 ]
机构：
[1] Vilnius Univ, Ctr Nanotechnol & Mat Sci, Fac Chem, LT-03225 Vilnius 6, Lithuania
[2] Vilnius Univ, Dept Phys Chem, Fac Chem, LT-03225 Vilnius, Lithuania
[3] Vilnius Univ, Lab Immunoanal & Nanotechnol, Inst Immunol, LT-08409 Vilnius 21, Lithuania
来源：
BIOELECTROCHEMISTRY | 2010年 / 79卷 / 01期
关键词：
Conducting polymers; Polypyrrole; EIS immunosensor; Nanotechnology; NanoBioTechnology; SURFACE-PLASMON RESONANCE; ANTIBODY-ANTIGEN INTERACTION; CONDUCTING-POLYMER; GLUCOSE-OXIDASE; REDOX ENZYME; IN-SITU; PROTEIN; BIOSENSORS; ELECTRODE; SENSORS;
D O I：
10.1016/j.bioelechem.2009.09.013
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Polypyrrole (Ppy) has been shown as a matrix for label-free electrochemical immunosensor based on electrochemical impedance spectroscopy (EIS) measurements. The immunosensing system model presented here was based on bovine leukemia virus (BLV) protein (gp51) entrapped within electrochemically-synthesized polypyrrole (Ppy/gp51). This Ppy/gp51 layer interacted with antibodies against gp51 (anti-gp51-Ab) that are present in significant concentration in the blood serum of BLV infected cattle. After this interaction protein complex (Ppy/gp51/anti-gp51-Ab) was formed. The horseradish peroxidase (HRP) labeled secondary antibodies (Ab*) against anti-gp51-Ab were applied as agents interacting with Ppy/gp51/anti-gp51-Ab and forming the large protein complex (Ppy/gp51/anti-gp51-Ab/Ab*). The EIS study was performed for electrodes modified with different Ppy layers described here and an optimal equivalent circuit was adopted for evaluation of EIS spectra, it was a major outcome of this study. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：11 / 16
页数：6
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