Triton X-100 as the "Short-Chain Lipid" Improves the Magnetic Alignment and Stability of Membrane Proteins in Phosphatidylcholine Bilayers for Oriented-Sample Solid-State NMR Spectroscopy

被引：41

作者：

Park, Sang Ho ^{[1
]}

Opella, Stanley J. ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 36期

基金：

美国国家卫生研究院;

关键词：

DIPOLAR COUPLINGS; C-13; NMR;

D O I：

10.1021/ja1055565

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A mixture of phospholipids and Triton X-100 in a molar ratio of 5:1 forms well-aligned and stable bilayers that give superior solid-state NMR spectra of proteins. In a comparison, the oriented-sample (OS) solid-state NMR spectrum of Pf1 coat protein in aligned phospholipid bilayers displayed better resolution than the equivalent solution NMR spectrum of the same protein in micelles. Both samples and experimental parameters were fully optimized.

引用

页码：12552 / 12553

页数：2

共 18 条

[1] Measurement of residual dipolar couplings of macromolecules aligned in the nematic phase of a colloidal suspension of rod-shaped viruses [J].

Clore, GM ;

Starich, MR ;

Gronenborn, AM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (40) :10571-10572

[2] High-resolution NMR spectroscopy of membrane proteins in aligned bicelles [J].

De Angelis, AA ;

Nevzorov, AA ;

Park, SH ;

Howell, SC ;

Mrse, AA ;

Opella, SJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (47) :15340-15341

[3] Structure determination of a membrane protein with two trans-membrane helices in aligned phospholipid bicelles by solid-state NMR spectroscopy [J].

De Angelis, Anna A. ;

Howell, Stanley C. ;

Nevzorov, Alexander A. ;

Opella, Stanley J. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (37) :12256-12267

[4] A Modified Alderman-Grant Coil makes possible an efficient cross-coil probe for high field solid-state NMR of lossy biological samples [J].

Grant, Christopher V. ;

Yang, Yuan ;

Glibowicka, Mira ;

Wu, Chin H. ;

Park, Sang Ho ;

Deber, Charles M. ;

Opella, Stanley J. .

JOURNAL OF MAGNETIC RESONANCE, 2009, 201 (01) :87-92

[5]

Hansen MR, 2000, METHOD ENZYMOL, V317, P220

[6] SEPARATED LOCAL FIELD SPECTRA IN NMR - DETERMINATION OF STRUCTURE OF SOLIDS [J].

HESTER, RK ;

ACKERMAN, JL ;

NEFF, BL ;

WAUGH, JS .

PHYSICAL REVIEW LETTERS, 1976, 36 (18) :1081-1083

[7] Weak alignment of membrane proteins in stressed polyacrylamide gels [J].

Jones, DH ;

Opella, SJ .

JOURNAL OF MAGNETIC RESONANCE, 2004, 171 (02) :258-269

[8] Selective averaging for high-resolution solid-state NMR spectroscopy of aligned samples [J].

Nevzorov, Alexander A. ;

Opella, Stanley J. .

JOURNAL OF MAGNETIC RESONANCE, 2007, 185 (01) :59-70

[9] 2-DIMENSIONAL C-13 NMR OF HIGHLY ORIENTED POLYETHYLENE [J].

OPELLA, SJ ;

WAUGH, JS .

JOURNAL OF CHEMICAL PHYSICS, 1977, 66 (11) :4919-4924

[10] Structure, dynamics, and assembly of filamentous bacteriophages by nuclear magnetic resonance spectroscopy [J].

Opella, Stanley J. ;

Zeri, Ana Carolina ;

Park, Sang Ho .

ANNUAL REVIEW OF PHYSICAL CHEMISTRY, 2008, 59 :635-657

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