Identifying QTLs for fire-blight resistance via a European pear (Pyrus communis L.) genetic linkage map

被引:78
|
作者
Dondini, L [1 ]
Pierantoni, L
Gaiotti, F
Chiodini, R
Tartarini, S
Bazzi, C
Sansavini, S
机构
[1] Univ Bologna, Dipartimento Colture Arboree, I-40126 Bologna, Italy
[2] Univ Bologna, Dipartimento Sci & Tecnol Agroambientali, I-40126 Bologna, Italy
关键词
Erwinia amylovora; molecular markers; Pyrus communis L; RGA;
D O I
10.1007/s11032-004-0505-y
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The existence of different levels of susceptibility to fire blight (Erwinia amylovora) in European pear (Pyrus communis L.) cultivars suggests that it is possible to identify QTLs related to resistance in pear germplasm. Given the polygenic nature of this trait, we designed two genetic maps of the parental lines 'Passe Crassane' (susceptible) and 'Harrow Sweet' (resistant) using SSRs, MFLPs, AFLPs, RGAs and AFLP-RGAs markers. RGA-related markers should theoretically map in chromosome regions coding for resistance genes. The 'Passe Crassane' map includes 155 loci, for a total length of 912 cM organised in 18 linkage groups, and the 'Harrow Sweet' map 156 loci, for a total length of 930 cM divided in 19 linkage groups; both maps have a good genome coverage when compared to the more detailed apple maps. Four putative QTLs related to fire blight resistance were identified in the map. A suite of molecular markers, including two AFLP-RGAs, capable of defining resistant and susceptible haplotypes in the analysed population was developed.
引用
收藏
页码:407 / 418
页数:12
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