Genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm revealed by RAPD markers

被引：45

作者：

Amadou, HI ^{[1
]}

Bebeli, PJ ^{[1
]}

Kaltsikes, PJ ^{[1
]}

机构：

[1] Agr Univ Athens, Dept Plant Breeding & Biometry, Athens 11855, Greece

来源：

GENOME | 2001年 / 44卷 / 06期

关键词：

germplasm; PCR; RAPD; Vigna subterranea;

D O I：

10.1139/gen-44-6-995

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm using 25 African accessions from the collection in the International Institute for Tropical Agriculture, Ibadan, Nigeria. Fifty random decamer primers were screened to assess their ability to detect polymorphism in bambara; 17 of them were selected for this study. Considerable genetic diversity was found among the V. subterranea accessions studied. The relationships among the 25 accessions were studied by cluster analysis. The dendrograms showed two main groups of accessions mainly along the lines of their geographic origin. It is concluded that RAPD can be used for germplasm classification in bambara groundnut and hence for improving this crop.

引用

页码：995 / 999

页数：5

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