DNA methylation;
CpG promoter microarray;
preeclampsia;
placenta;
GLOBAL DNA METHYLATION;
ASSOCIATION;
PATHOGENESIS;
EPIGENETICS;
EXPRESSION;
GENETICS;
GENES;
CELLS;
D O I:
10.3892/ijmm.2012.983
中图分类号:
R-3 [医学研究方法];
R3 [基础医学];
学科分类号:
1001 ;
摘要:
The development of preeclampsia (PE) seriously affects the health of the mother and the child, but the precise pathogenesis of PE remains elusive. The placenta is considered to play a key role and DNA methylation may be associated with altered placental development and function. The aim of this study was to perform a genome-wide analysis of the DNA methylation profile in placentas from pregnancies with severe preeclampsia. The authors analyzed normal and placental tissues with PE for aberrant DNA methylation using methylated DNA immunoprecipitation (MeDIP) and a human CpG island plus promoter microarray. The methylation status of identified candidate genes were validated by bisulfite sequencing PCR (BSP). Microarray analysis identified 296 genes that showed significantly aberrant DNA methylation in preeclampsia (PE). These genes were located more frequently in chromosome 1 (10.5%, P=0.005), chromosome 12 (8.1%, P=0.062) and chromosome 19 (7.4%, P=0.117). Functional analysis divided these genes into different functional networks. In addition, the methylation profile of six of these genes (CAPN2, EPHX2, ADORA2B, SOX7, CXCL1 and CDX1) in nine patients with PE was validated by BSP. This study demonstrated aberrant patterns of DNA methylation in PE, which may be involved in the pathophysiology of PE. Future work will assess the potential prognostic and therapeutic value for these findings in PE.
机构:
Univ Calgary, Fac Med, Dept Biochem & Mol Biol, Genes & Dev Res Grp, Calgary, AB T2N 4N1, CanadaUniv Calgary, Fac Med, Dept Biochem & Mol Biol, Genes & Dev Res Grp, Calgary, AB T2N 4N1, Canada
机构:
Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Gao, Wen-long
Li, Dong
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Li, Dong
Xiao, Zhong-xin
论文数: 0引用数: 0
h-index: 0
机构:
Capital Med Univ, Sch Publ Hlth & Family Med, Beijing, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Xiao, Zhong-xin
Liao, Qin-ping
论文数: 0引用数: 0
h-index: 0
机构:
Peking Univ, Hosp 1, Dept Obstet & Gynecol, Beijing 100871, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Liao, Qin-ping
Yang, Hui-xia
论文数: 0引用数: 0
h-index: 0
机构:
Peking Univ, Hosp 1, Dept Obstet & Gynecol, Beijing 100871, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Yang, Hui-xia
Li, Yu-xia
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Li, Yu-xia
Ji, Lei
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Ji, Lei
Wang, Yan-ling
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
机构:
Univ Calgary, Fac Med, Dept Biochem & Mol Biol, Genes & Dev Res Grp, Calgary, AB T2N 4N1, CanadaUniv Calgary, Fac Med, Dept Biochem & Mol Biol, Genes & Dev Res Grp, Calgary, AB T2N 4N1, Canada
机构:
Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Gao, Wen-long
Li, Dong
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Chinese Acad Sci, Grad Sch, Beijing 100101, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Li, Dong
Xiao, Zhong-xin
论文数: 0引用数: 0
h-index: 0
机构:
Capital Med Univ, Sch Publ Hlth & Family Med, Beijing, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Xiao, Zhong-xin
Liao, Qin-ping
论文数: 0引用数: 0
h-index: 0
机构:
Peking Univ, Hosp 1, Dept Obstet & Gynecol, Beijing 100871, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Liao, Qin-ping
Yang, Hui-xia
论文数: 0引用数: 0
h-index: 0
机构:
Peking Univ, Hosp 1, Dept Obstet & Gynecol, Beijing 100871, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Yang, Hui-xia
Li, Yu-xia
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Li, Yu-xia
Ji, Lei
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China
Ji, Lei
Wang, Yan-ling
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R ChinaChinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100101, Peoples R China