Nucleoside triphosphate diphosphohydrolases (NTPDase) inhibitory activity of some medicinal plants.

被引：0
作者：
Ashraf, Muhammad [1 ]
Shah, Syed Muhammad Ali [2 ]
Ahmad, Irshad [1 ]
Ahmad, Saeed [1 ]
Arshad, Shafia [2 ]
Ahmad, Khalil [2 ]
Nasim, Faiz-ul-Hassan [3 ]
机构：
[1] Islamia Univ Bahawalpur, Fac Pharm & Alternat Med, Dept Pharm, Bahawalpur, Pakistan
[2] Islamia Univ Bahawalpur, Fac Pharm & Alternat Med, Univ Coll Convent Med, Bahawalpur, Pakistan
[3] Islamia Univ Bahawalpur, Dept Chem, Bahawalpur, Pakistan
来源：
JOURNAL OF MEDICINAL PLANTS RESEARCH | 2011年 / 5卷 / 10期
关键词：
Nucleoside triphosphate diphosphohydrolases; NTPDases; medicinal plants; ECTO-ATPASE;
D O I：
暂无
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Nucleoside triphosphate diphosphohydrolasess (NTPDases, EC 3.6.1.5) are well characterized ectoenzymes which are involved in the hydrolysis of ATP and ADP resulting in the formation of nucleoside monophosphates and orthophosphate. By hydrolyzing circulating ATP to ADP to AMP, NTPDases play a crucial role in preventing the activation of platelets and their aggregation. NTPDase inhibitors therefore are currently target for various cardiovascular diseases and some types of cancer. In search for new enzyme inhibitors of therapeutic importance, the present study was conducted to find out the inhibitors of NTPDase from methanolic extracts of 50 medicinal plants used in folk medicine. Data shows that only 6 plants exhibited anti-NTPDase activity above 50% with crude enzyme preparation of chicken liver at 125 mu g plant extract. These plants included, Cymbopogan jawarancusa, (51.16 +/- 4.81%), Asparagus officinalis (58.24 +/- 0.93%), Tribulus terrestris (50.84 +/- 6.19%), Rubia cordizolla (53.11 +/- 3.49%), Echimops echimatus (51.48 +/- 6.29%) Portulaca oleracea (52.79 +/- 0.10%). These studies are priliminary findings and further work is required to explore these plants using purified enzyme preparations.
引用
收藏
页码：2090 / 2094
页数：5
相关论文
共 17 条
[1]  
Aaron J, 2005, SEMIN THROMB HEMOST, V31, P234
[2]   Ecto-nucleotidases of the CD39/NTPDase family modulate platelet activation and thrombus fori-nation: Potential as therapeutic targets [J].
Atkinson, B ;
Dwyer, K ;
Enjyoji, K ;
Robson, SC .
BLOOD CELLS MOLECULES AND DISEASES, 2006, 36 (02) :217-222
[3]  
Bigonesse F, 2004, SEVINGY J BIOCH, V43, P5511
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   A DIRECT COLORIMETRIC ASSAY FOR CA-2+-STIMULATED ATPASE ACTIVITY [J].
CHAN, KM ;
DELFERT, D ;
JUNGER, KD .
ANALYTICAL BIOCHEMISTRY, 1986, 157 (02) :375-380
[6]   PHARMACOLOGICAL-ANALYSIS AND BIOCHEMICAL-ANALYSIS OF FPL-67156, A NOVEL, SELECTIVE INHIBITOR OF ECTO-ATPASE [J].
CRACK, BE ;
POLLARD, CE ;
BEUKERS, MW ;
ROBERTS, SM ;
HUNT, SF ;
INGALL, AH ;
MCKECHNIE, KCW ;
IJZERMAN, TP ;
LEFF, P .
BRITISH JOURNAL OF PHARMACOLOGY, 1995, 114 (02) :475-481
[7]   Inhibition of NTPDase, 5′-nucleotidase, Na+/K+-ATPase and acetylcholinesterase activities by subehronic treatment with Casearia sylvestris [J].
da Silva, A. C. ;
Balz, D. ;
de Souza, J. B. D'A. ;
Morsch, V. M. ;
Correa, M. C. ;
Zanetti, G. D. ;
Manfron, M. P. ;
Schetinger, M. R. C. .
PHYTOMEDICINE, 2006, 13 (07) :509-514
[8]   Site-directed mutagenesis of human endothelial cell ecto-ADPase/soluble CD39: Requirement of glutamate 174 and serine 218 for enzyme activity and inhibition of platelet recruitment [J].
Drosopoulos, JHF ;
Broekman, MJ ;
Islam, N ;
Maliszewski, CR ;
Gayle, RB ;
Marcus, AJ .
BIOCHEMISTRY, 2000, 39 (23) :6936-6943
[9]   Purine signaling and potential new therapeutic approach: Possible outcomes of NTPDase inhibition [J].
Gendron, FP ;
Benrezzak, O ;
Krugh, BW ;
Kong, Q ;
Weisman, GA ;
Beaudoin, AR .
CURRENT DRUG TARGETS, 2002, 3 (03) :229-245
[10]  
Hiroaki H, 1990, Nucleic Acids Symp Ser, P53
12