Purification and Characterization of Extracellular, Polyextremophilic α-amylase Obtained from Halophilic Engyodontium album

被引：15

作者：

Ali, Imran ^{[1
,2
]}

Akbar, Ali ^{[3
]}

Anwar, Muhammad ^{[2
]}

Yanwisetpakdee, Benjawan ^{[1
]}

Prasongsuk, Sehanat ^{[1
]}

Lotrakul, Pongtharin ^{[1
]}

Punnapayak, Hunsa ^{[1
]}

机构：

[1] Chulalongkorn Univ, Fac Sci, Dept Bot, Plant Biomass Utilizat Res Unit, Bangkok 10330, Thailand

[2] Univ Balochistan, Inst Biochem, Quetta 87300, Pakistan

[3] Asian Inst Technol, Sch Environm Resources & Dev, Food Engn & Bioprocess Technol, Klongluang 12120, Pathumthani, Thailand

来源：

IRANIAN JOURNAL OF BIOTECHNOLOGY | 2014年 / 12卷 / 04期

关键词：

alpha-amylase; Engyodontium album; Extremozyme; Halophilic fungi; BIOCHEMICAL-CHARACTERIZATION; ASPERGILLUS-NIGER; INDUSTRIAL; IDENTIFICATION; EXPRESSION; SURFACTANT; CLONING;

D O I：

10.15171/ijb.1155

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: alpha-Amylases (EC 3.2.1.1) are covering approximately 25% of total enzyme market and are frequently used in food, pharmaceutical and detergent industries. Objectives: The first ever detailed characterization of amylase from any halophilic Engyodontium album is presented. Materials and Methods: An extracellular alpha-amylase was studied from halophilic E. album TISTR 3645. The enzyme was extracted and purified by column chromatography. SDS-PAGE was performed to find the molecular weight of the enzyme. The effects of pH, temperature and salinity on the isolated enzyme were determined. The effects of various additives on enzyme were studied. Results: The molecular weight of the amylase was 50 kDa. The enzyme specific activity was 132.17 U.mg(-1) with V-max and K-m values of 15.36 U.mg(-1) and 6.28 mg.ml(-1), respectively. The optimum enzyme activities were found at pH 9.0, 60 degrees C and 30% (w/v) NaCl. BaCl2, CaCl2, HgCl2 and MgCl2 improved amylase activity. beta-mercaptoethanol, EDTA, FeCl2 and ZnCl2 decreased enzyme activity. Conclusions: Polyextremophilic characteristics of alpha-amylase from halophilic E. album TISTR 3645 were revealed during the characterization studies, demonstrating promising features, making it a useful candidate for various industries.

引用

页码：35 / 40

页数：6

共 36 条

[1]

Ali I, 2013, LUJST, V2, P47

[2]

Ali I, 2014, BIOMED RES INT

[3]

Ali I, 2014, PAK J BOT, V46, P983

[4] Identification, phylogenetic analysis and characterization of obligate halophilic fungi isolated from a man-made solar saltern in Phetchaburi province, Thailand [J].