Expression in a wine yeast strain of the Aspergillus niger abfB gene

被引：33

作者：

SanchezTorres, P ^{[1
]}

GonzalezCandelas, L ^{[1
]}

Ramon, D ^{[1
]}

机构：

[1] UNIV VALENCIA,CSIC,INST AGROQUIM & TECNOL ALIMENTOS,DEPT BIOTECNOL ALIMENTOS,E-46100 BURJASSOT,VALENCIA,SPAIN

来源：

FEMS MICROBIOLOGY LETTERS | 1996年 / 145卷 / 02期

关键词：

wine yeast; arabinofuranosidase; Aspergillus niger; abfB;

D O I：

10.1016/S0378-1097(96)00406-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A recombinant wine yeast strain has been constructed expressing the gene coding for alpha-L-arabinofuranosidase B from Aspergillus niger under the control of the yeast actin gene promoter. The protein is efficiently secreted by the recombinant yeast, allowing its purification and characterisation. The heterologous alpha-L-arabinofuranosidase B Shows similar physicochemical properties to the native enzyme. The wine produced in microvinification experiments using the recombinant yeast presents the same oenological characteristics as obtained with the untransformed strain. The alpha-L-arabinofuranosidase B protein is detected throughout the fermentation.

引用

页码：189 / 194

页数：6

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