Extracellular polysaccharides of modified strains of Erwinia spp.

被引：10

作者：

Yang, BY ^{[1
]}

Brand, JM ^{[1
]}

Gray, JSS ^{[1
]}

Montgomery, R ^{[1
]}

机构：

[1] Univ Iowa, Coll Med, Dept Biochem, Iowa City, IA 52242 USA

来源：

CARBOHYDRATE RESEARCH | 2001年 / 333卷 / 04期

关键词：

Erwinia spp; streptomycin; extracellular polysaccharide; structure; ribotyping;

D O I：

10.1016/S0008-6215(01)00155-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of the extracellular polysaccharide (EPS) produced by Erwinia chrysanthemi strain A2148 has been determined using low pressure size-exclusion and anion-exchange chromatographies, high pH anion-exchange chromatography, glycosyl-linkage analysis, and 1D H-1 NMR spectroscopy. The polysaccharide is structurally similar, if not identical, to the EPS produced by E. chrysanthemi strain A350. A streptomycin-resistant strain of E. chrysanthemi Ech6 (Ech6S(+)) has been generated and has an elevated production of EPS, as does a streptomycin-resistant strain (Ech9Sm6) of E. chrysanthemi Ech9. These modified E. chrysanthemi spp. have been ribotyped and found to be closely related to their parent strains. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：295 / 302

页数：8