Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

被引：10

作者：

Kuwayama, Masaru ^{[1
]}

Shigemoto, Naoki ^{[1
]}

Oohara, Sachiko ^{[1
]}

Tanizawa, Yukie ^{[1
]}

Yamada, Hiroko ^{[1
]}

Takeda, Yoshihiro ^{[1
]}

Matsuo, Takeshi ^{[1
]}

Fukuda, Shinji ^{[1
]}

机构：

[1] Hiroshima Prefectural Technol Res Inst, Ctr Publ Hlth & Environm, Minami Ku, Hiroshima 7340007, Japan

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2011年 / 86卷 / 01期

关键词：

Multiplex; PCR; E; coli; DYES;

D O I：

10.1016/j.mimet.2011.04.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed simultaneous detection of eight genes associated with the five categories of diarrheagenic Escherichia coli by the multiplex PCR assay with Alexa Fluor-labeled primers. This assay can easily distinguish eight genes based on the size and color of amplified products without gel staining. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：119 / 120

页数：2

共 10 条

[1] Validation of Five-Colony Pool Analysis Using Multiplex Real-Time PCR for Detection of Diarrheagenic Escherichia coli [J].

Barletta, F. ;

Ochoa, T. J. ;

Ecker, L. ;

Gil, A. I. ;

Lanata, C. F. ;

Cleary, T. G. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (06) :1915-1917

[2] Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: Fluorescence of the dyes and their bioconjugates [J].

Berlier, JE ;

Rothe, A ;

Buller, G ;

Bradford, J ;

Gray, DR ;

Filanoski, BJ ;

Telford, WG ;

Yue, S ;

Liu, JX ;

Cheung, CY ;

Chang, W ;

Hirsch, JD ;

Beechem, JM ;

Haugland, RP ;

Haugland, RP .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2003, 51 (12) :1699-1712

[3] Phylogenetic analysis of enteroaggregative and diffusely adherent Escherichia coli [J].

Czeczulin, JR ;

Whittam, TS ;

Henderson, IR ;

Navarro-Garcia, F ;

Nataro, JP .

INFECTION AND IMMUNITY, 1999, 67 (06) :2692-2699

[4]

Fujioka M, 2009, JPN J INFECT DIS, V62, P476

[5] A set of novel multiplex Taqman real-time PCRs for the detection of diarrhoeagenic Escherichia coli and its use in determining the prevalence of EPEC and EAEC in a university hospital [J].

Hardegen, Christoph ;

Messler, Sabine ;

Henrich, Birgit ;

Pfeffer, Klaus ;

Wuerthner, Jens ;

MacKenzie, Colin R. .

ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS, 2010, 9

[6]

Nataro JP, 1998, CLIN MICROBIOL REV, V11, P403

[7] Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates [J].

Panchuk-Voloshina, N ;

Haugland, RP ;

Bishop-Stewart, J ;

Bhalgat, MK ;

Millard, PJ ;

Mao, F ;

Leung, WY ;

Haugland, RP .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1999, 47 (09) :1179-1188

[8] Development of Multiplex PCR Assays for Detection of Enterotoxigenic Escherichia coli Colonization Factors and Toxins [J].

Rodas, Claudia ;

Iniguez, Volga ;

Qadri, Firdausi ;

Wiklund, Gudrun ;

Svennerholm, Ann-Mari ;

Sjoeling, Asa .

JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (04) :1218-1220

[9] MULTIPLEX PCR ASSAY AND SIMPLE PREPARATION METHOD FOR STOOL SPECIMENS DETECT ENTEROTOXIGENIC ESCHERICHIA-COLI DNA DURING COURSE OF INFECTION [J].

STACYPHIPPS, S ;

MECCA, JJ ;

WEISS, JB .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (05) :1054-1059

[10] Characterization of enteropathogenic and enteroaggregative Escherichia coli isolated from diarrheal outbreaks [J].

Yatsuyanagi, J ;

Saito, S ;

Sato, H ;

Miyajima, Y ;

Amano, KI ;

Enomoto, K .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (01) :294-297

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