Aurora kinase A induces migration and invasion by inducing epithelial-to-mesenchymal transition in colon cancer cells

被引：10

作者：

Hong, On-Yu ^{[1
]}

Kang, Sang Yull ^{[2
,3
]}

Noh, Eun-Mi ^{[1
]}

Yu, Hong-Nu ^{[1
]}

Jang, Hye-Yeon ^{[1
]}

Kim, Seong-Hun ^{[4
]}

Hong, Jingyu ^{[5
]}

Chung, Eun Yong ^{[5
]}

Kim, Jong-Suk ^{[1
]}

机构：

[1] Jeonbuk Natl Univ, Inst Med Sci, Dept Biochem, Med Sch, Jeonju 54907, South Korea

[2] Jeonbuk Natl Univ, Dept Surg, Res Inst Clin Med, Jeonju 54907, South Korea

[3] Jeonbuk Natl Univ Hosp, Biomed Res Inst, Jeonju 54907, South Korea

[4] Jeonbuk Natl Univ, Res Inst Clin Med, Dept Internal Med, Div Gastroenterol,Med Sch & Hosp, Jeonju 54907, South Korea

[5] Catholic Univ Korea, Coll Med, Dept Anesthesiol & Pain Med, Bucheon St Marys Hosp, Seoul 06591, South Korea

来源：

BMB REPORTS | 2022年 / 55卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Aurora kinase A; Colon cancer; Epithelia-to-mesenchymal transition; Matrix metalloproteinase-9; Protein kinase C; NF-KAPPA-B; MATRIX METALLOPROTEINASES; EXPRESSION; PROTEIN; TARGET; SNAIL; CHFR;

D O I：

10.5483/BMBRep.2022.55.2.169

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aurora kinase is a family of serine/threonine kinases intimately associated with mitotic progression and the development of human cancers. Studies have shown that aurora kinases are important for the protein kinase C (PKC)-induced invasion of colon cancer cells. Recent studies have shown that aurora kinase A promotes distant metastasis by inducing epithelial-to-mesenchymal transition (EMT) in colon cancer cells. However, the role of aurora kinase A in colon cancer metastasis remains unclear. In this study, we investigated the effects of aurora kinase A on PKC-induced cell invasion, migration, and EMT in human SW480 colon cancer cells. Treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) changed the expression levels of EMT markers, increasing alpha-SMA, vimentin, and MMP-9 expression and decreasing E-cadherin expression, with changes in cell morphology. TPA treatment induced EMT in a PKC-dependent manner. More-over, the inhibition of aurora kinase A by siRNAs and inhibitors (reversine and VX-680) suppressed TPA-induced cell invasion, migration, and EMT in SW480 human colon cells. Inhibition of aurora kinase A blocked TPA-induced vimentin and MMP-9 expression, and decreased E-cadherin expression. Furthermore, the knockdown of aurora kinase A decreased the transcriptional activity of NF-kappa B and AP-1 in PKC-stimulated SW480 cells. These findings indicate that aurora kinase A induces migration and invasion by inducing EMT in SW480 colon cancer cells. To the best of our knowledge, this is the first study that showed aurora kinase A is a key molecule in PKC-induced metastasis in colon cancer cells.

引用

页码：87 / 91

页数：5

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