Aurora kinase A induces migration and invasion by inducing epithelial-to-mesenchymal transition in colon cancer cells

被引：9
作者：
Hong, On-Yu [1 ]
Kang, Sang Yull [2 ,3 ]
Noh, Eun-Mi [1 ]
Yu, Hong-Nu [1 ]
Jang, Hye-Yeon [1 ]
Kim, Seong-Hun [4 ]
Hong, Jingyu [5 ]
Chung, Eun Yong [5 ]
Kim, Jong-Suk [1 ]
机构：
[1] Jeonbuk Natl Univ, Inst Med Sci, Dept Biochem, Med Sch, Jeonju 54907, South Korea
[2] Jeonbuk Natl Univ, Dept Surg, Res Inst Clin Med, Jeonju 54907, South Korea
[3] Jeonbuk Natl Univ Hosp, Biomed Res Inst, Jeonju 54907, South Korea
[4] Jeonbuk Natl Univ, Res Inst Clin Med, Dept Internal Med, Div Gastroenterol,Med Sch & Hosp, Jeonju 54907, South Korea
[5] Catholic Univ Korea, Coll Med, Dept Anesthesiol & Pain Med, Bucheon St Marys Hosp, Seoul 06591, South Korea
来源：
BMB REPORTS | 2022年 / 55卷 / 02期
基金：
新加坡国家研究基金会;
关键词：
Aurora kinase A; Colon cancer; Epithelia-to-mesenchymal transition; Matrix metalloproteinase-9; Protein kinase C; NF-KAPPA-B; MATRIX METALLOPROTEINASES; EXPRESSION; PROTEIN; TARGET; SNAIL; CHFR;
D O I：
10.5483/BMBRep.2022.55.2.169
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Aurora kinase is a family of serine/threonine kinases intimately associated with mitotic progression and the development of human cancers. Studies have shown that aurora kinases are important for the protein kinase C (PKC)-induced invasion of colon cancer cells. Recent studies have shown that aurora kinase A promotes distant metastasis by inducing epithelial-to-mesenchymal transition (EMT) in colon cancer cells. However, the role of aurora kinase A in colon cancer metastasis remains unclear. In this study, we investigated the effects of aurora kinase A on PKC-induced cell invasion, migration, and EMT in human SW480 colon cancer cells. Treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) changed the expression levels of EMT markers, increasing alpha-SMA, vimentin, and MMP-9 expression and decreasing E-cadherin expression, with changes in cell morphology. TPA treatment induced EMT in a PKC-dependent manner. More-over, the inhibition of aurora kinase A by siRNAs and inhibitors (reversine and VX-680) suppressed TPA-induced cell invasion, migration, and EMT in SW480 human colon cells. Inhibition of aurora kinase A blocked TPA-induced vimentin and MMP-9 expression, and decreased E-cadherin expression. Furthermore, the knockdown of aurora kinase A decreased the transcriptional activity of NF-kappa B and AP-1 in PKC-stimulated SW480 cells. These findings indicate that aurora kinase A induces migration and invasion by inducing EMT in SW480 colon cancer cells. To the best of our knowledge, this is the first study that showed aurora kinase A is a key molecule in PKC-induced metastasis in colon cancer cells.
引用
收藏
页码：87 / 91
页数：5
相关论文
共 26 条
  • [1] Fra-1/AP-1 induces EMT in mammary epithelial cells by modulating Zeb1/2 and TGFβ expression
    Bakiri, L.
    Macho-Maschler, S.
    Custic, I.
    Niemiec, J.
    Guio-Carrion, A.
    Hasenfuss, S. C.
    Eger, A.
    Mueller, M.
    Beug, H.
    Wagner, E. F.
    [J]. CELL DEATH AND DIFFERENTIATION, 2015, 22 (02) : 336 - 350
  • [2] The transcription factor Snail is a repressor of E-cadherin gene expression in epithelial tumour cells
    Batlle, E
    Sancho, E
    Franci, C
    Domínguez, D
    Monfar, M
    Baulida, J
    de Herreros, AG
    [J]. NATURE CELL BIOLOGY, 2000, 2 (02) : 84 - 89
  • [3] New facets of matrix metalloproteinases MMP-2 and MMP-9 as cell surface transducers: Outside-in signaling and relationship to tumor progression
    Bauvois, Brigitte
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 2012, 1825 (01): : 29 - 36
  • [4] Aurora kinases: New targets for cancer therapy
    Carvajal, Richard D.
    Tse, Archie
    Schwartz, Gary K.
    [J]. CLINICAL CANCER RESEARCH, 2006, 12 (23) : 6869 - 6875
  • [5] The mitotic kinase Aurora-A promotes distant metastases by inducing epithelial-to-mesenchymal transition in ERα+ breast cancer cells
    D'Assoro, A. B.
    Liu, T.
    Quatraro, C.
    Amato, A.
    Opyrchal, M.
    Leontovich, A.
    Ikeda, Y.
    Ohmine, S.
    Lingle, W.
    Suman, V.
    Ecsedy, J.
    Iankov, I.
    Di Leonardo, A.
    Ayers-Ringler, J.
    Degnim, A.
    Billadeau, D.
    McCubrey, J.
    Ingle, J.
    Salisbury, J. L.
    Galanis, E.
    [J]. ONCOGENE, 2014, 33 (05) : 599 - 610
  • [6] Aurora-A Kinase as a Promising Therapeutic Target in Cancer
    D'Assoro, Antonino B.
    Haddad, Tufia
    Galanis, Evanthia
    [J]. FRONTIERS IN ONCOLOGY, 2016, 5
  • [7] Targeting AURKA in Cancer: molecular mechanisms and opportunities for Cancer therapy
    Du, Ruijuan
    Huang, Chuntian
    Liu, Kangdong
    Li, Xiang
    Dong, Zigang
    [J]. MOLECULAR CANCER, 2021, 20 (01)
  • [8] Aurora kinase A (AURKA) expression in colorectal cancer liver metastasis is associated with poor prognosis
    Goos, J. A. C. M.
    Coupe, V. M. H.
    Diosdado, B.
    Diemen, P. M. Delis-Van
    Karga, C.
    Belien, J. A. M.
    Carvalho, B.
    van den Tol, M. P.
    Verheul, H. M. W.
    Geldof, A. A.
    Meijer, G. A.
    Hoekstra, O. S.
    Fijneman, R. J. A.
    [J]. BRITISH JOURNAL OF CANCER, 2013, 109 (09) : 2445 - 2452
  • [9] Global Patterns of Cancer Incidence and Mortality Rates and Trends
    Jemal, Ahmedin
    Center, Melissa M.
    DeSantis, Carol
    Ward, Elizabeth M.
    [J]. CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION, 2010, 19 (08) : 1893 - 1907
  • [10] Activation of NF-κB by Akt upregulates Snail expression and induces epithelium mesenchyme transition
    Julien, S.
    Puig, I.
    Caretti, E.
    Bonaventure, J.
    Nelles, L.
    van Roy, F.
    Dargemont, C.
    Garcia de Herreros, A.
    Bellacosa, A.
    Larue, L.
    [J]. ONCOGENE, 2007, 26 (53) : 7445 - 7456
123