Filimonas effusa sp. nov., isolated from a freshwater river

Strain TTM-71(T), isolated from a freshwater river in Taiwan, was characterized using a polyphasic taxonomic approach. Phylogenetic analyses based on 165 rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that strain TTM-71(T) is affiliated with species in the genus Filimonas. The 165 rRNA gene sequence similarity indicated that strain TTM-71(T) is closely related to species within the genus Filimonas (94.7-95.5% sequence similarity) and had a high sequence similarity with Filimonas endophytica SR 2-06(T) (95.5 %). Strain TTM-71(T) showed 70.3% average nucleotide identity and 24.9% digital DNA-DNA hybridization identity with Filimonas lacunae YT21(T). Cells were Gram-stain-negative, aerobic, motile by gliding, rod-shaped and formed beige-colored colonies. Optimal growth occurred at 20 degrees C, pH 8, and in the presence of 0.5% NaCl. The major fatty acids of strain TTM-71(T) were iso-C-15:0, iso-C-15:1 G and summed feature 3 (comprising C-16:1 omega 7c and/or C-16:1 omega 6c). The predominant hydroxy fatty acid was iso-C-1(7:)0 3-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, four uncharacterized aminophospholipids, one uncharacterized aminolipid, one uncharacterized phospholipid and one uncharacterized lipid. The predominant polyamine was homospermidine. The only isoprenoid quinone was MK-7. Genomic DNA G+C content was 45.6 mol%. On the basis of the polyphasic evidence presented, strain TTM-71T is considered to represent a novel species of the genus Filimonas, for which the name Filimonas effusa sp. nov. is proposed. The type strain is TTM-71(T) (=BCRC 81160(T) =LMG 31017(T)=KCTC 62871(T)).