DNA-based markers for a recessive gene conferring anthracnose resistance in sorghum

被引：32
|
作者：
Boora, KS
Frederiksen, R
Magill, C [1 ]
机构：
[1] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA
[2] Haryana Agr Univ, Dept Biotechnol & Mol Biol, Hisar 125004, Haryana, India
来源：
CROP SCIENCE | 1998年 / 38卷 / 06期
关键词：
D O I：
10.2135/cropsci1998.0011183X003800060048x
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Resistance to anthracnose in sorghum [Sorghum bicolor (L.) Moench] accession SC326-6 was found to segregate as a simple recessive trait in a cross to the susceptible cultivar BTx623, F3 progeny tests following self pollination of 115 F-2 individuals identified homozygous resistant and susceptible F-2 plants for use in bulked-segregant analysis. DNA from the parental cultivars and the bulks was screened by PCR amplification with 300 RAPD (random amplified polymorphic DNA) primers. Two RAPD primers amplified a sequence that co-segregated with the recessive resistance allele, while another amplified a band linked to the susceptible allele.
引用
收藏
页码：1708 / 1709
页数：2
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