Programmed cell death during secondary xylem differentiation in Eucommia ulmoides

被引：0

作者：

Cao, J ^{[1
]}

He, XQ ^{[1
]}

Wang, YQ ^{[1
]}

Sodmergen ^{[1
]}

Ciu, KM ^{[1
]}

机构：

[1] Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China

来源：

ACTA BOTANICA SINICA | 2003年 / 45卷 / 12期

关键词：

caspase-like protease; DNA fragmentation; Eucommia ulmoides; poly (ADP-ribose) polymerase; programmed cell death; secondary xylem differentiation;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Programmed cell death (PCD) during secondary xylem differentiation in Eucommia ulmoides Oliv. was examined using electron microscopy and by investigation of DNA fragmentation and degradation of caspase-like proteases (CLPs). DNA ladders were detected in developing secondary xylem by gel electrophoresis. DNA fragmentation was further confirmed by using the TdT-mediated dUTP nick-end labeling (TUNEL) method. Western blotting analysis showed that CLPs (caspase-8- and caspase-3-like proteases) and PARP (poly (ADP-ribose) polymerase) were degraded during secondary xylem differentiation. The results thus indicated that secondary xylem differentiation in E ulmoides was a typical process of PCD and the degradation of CLPs might be a constitutive PCD event during secondary xylem differentiation.

引用

页码：1465 / 1474

页数：10

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