Method of of aerobic-anaerobic bioremediation of a raised bog in Western Siberia affected by old oil pollution. A pilot test

The aerobic-anaerobic method of bioremediation was used in Western Siberia on an extreme site of a raised bog polluted with oil 20 years ago. The area was covered with an asphalt crust that can withstand a weight up to 60 kg without being destroyed. The crust was up to 1.5 cm thick; the underlying heavily oil-polluted moss was 15-25 cm. This area was divided into three plots (25 x 30 m(2)). The concentration of total petroleum hydrocarbons (TPH) was 446.7-526.8 g/kg DM. The oil-oxidizing preparation Rhoder and a fertilizer were applied three times to the surface of the first plot by sprinkling; the electron acceptor was introduced into the moss together with the Rhoder under pressure to 15 cm. On the second plot indigenous microorganisms were activated with a fertilizer three times. The third plot was used as a negative control. As a result TPH concentration decreased by 23.0% in the layer of 0-10 cm and by 72.6% in the layer of 10-25 cm on the first plot. On the second plot (the activation of indigenous microflora) the TPH decreased by 28.7% only in the layer of 10-25 cm. The HC concentration in the control plot did not decrease at all. (C) 2016 Elsevier Ltd. All rights reserved.