Gut Microbial Diversity Is Reduced in Smokers with Crohn's Disease

被引:84
作者
Opstelten, Jorrit L. [1 ]
Plassais, Jonathan [2 ]
van Mil, Saskia W. C. [3 ]
Achouri, Emna [2 ]
Pichaud, Matthieu [2 ]
Siersema, Peter D. [1 ,4 ]
Oldenburg, Bas [1 ]
Cervino, Alessandra C. L. [2 ]
机构
[1] Univ Med Ctr Utrecht, Dept Gastroenterol & Hepatol, Internal Post F02-618,POB 85500, NL-3508 GA Utrecht, Netherlands
[2] Enterome, Paris, France
[3] Univ Med Ctr Utrecht, Ctr Mol Med, Utrecht, Netherlands
[4] Radboud Univ Nijmegen, Med Ctr, Dept Gastroenterol & Hepatol, Nijmegen, Netherlands
关键词
smoking; microbiota; Crohn's disease; inflammatory bowel disease; sequencing; INFLAMMATORY-BOWEL-DISEASE; SP NOV; GEN; NOV; INTESTINAL MICROBIOME; SMOKING-CESSATION; FECAL MICROBIOTA; IMPACT; BACTERIA; INSIGHTS; COLITIS;
D O I
10.1097/MIB.0000000000000875
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background: Smoking has a negative impact on Crohn's disease (CD), but the mechanisms underlying this association are unclear. We compared the gut microbiota composition of smoking with nonsmoking patients with CD using a metagenomic approach. Methods: Stool samples and clinical data were collected from current smokers and nonsmokers with CD from France and the Netherlands, matched for country, gender, age, disease activity, and body mass index. Fecal DNA was sequenced on an Illumina HiSeq 2500. On average, 40 million paired-end reads were generated per sample. Gene richness and the Shannon index were computed to assess microbial diversity. Wilcoxon's signed-rank tests for paired samples were performed to detect differences between the 2 groups. Results: In total, 21 smoking and 21 nonsmoking patients with CD were included. Compared with nonsmoking patients, gut microbial gene richness (P = 0.01), genus diversity (P < 0.01), and species diversity (P = 0.01) were decreased in smoking patients. This was accompanied by a reduced relative abundance of the genera Collinsella (P = 0.02), Enterorhabdus (P = 0.02), and Gordonibacter (P = 0.02) in smokers. No statistically significant differences at the species level were observed, although smokers had lower proportions of Faecalibacterium prausnitzii (P = 0.10). Conclusions: Gut microbial diversity is reduced in smokers with CD compared with nonsmokers with CD. The microbial profile differs between these groups at the genus level. Future studies should evaluate whether intestinal microbes mediate the adverse effects of smoking in CD.
引用
收藏
页码:2070 / 2077
页数:8
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