RNA polymerase II is required for spatial chromatin reorganization following exit from mitosis

被引：62

作者：

Zhang, Shu ^{[1
]}

Uebelmesser, Nadine ^{[1
]}

Josipovic, Natasa ^{[1
]}

Forte, Giada ^{[2
]}

Slotman, Johan A. ^{[3
]}

Chiang, Michael ^{[2
]}

Gothe, Henrike ^{[4
]}

Gusmao, Eduardo Gade ^{[1
]}

Becker, Christian ^{[5
]}

Altmueller, Janine ^{[5
]}

Houtsmuller, Adriaan B. ^{[3
]}

Roukos, Vassilis ^{[4
]}

Wendt, Kerstin S. ^{[6
]}

Marenduzzo, Davide ^{[2
]}

Papantonis, Argyris ^{[1
,7
]}

机构：

[1] Univ Med Ctr Gottingen, Inst Pathol, D-37075 Gottingen, Germany

[2] Univ Edinburgh, Sch Phys & Astron, Edinburgh EH9 3FD, Midlothian, Scotland

[3] Erasmus MC, Opt Imaging Ctr, NL-3015 GD Rotterdam, Netherlands

[4] Inst Mol Biol, D-55128 Mainz, Germany

[5] Univ Cologne, Cologne Ctr Genom, D-50931 Cologne, Germany

[6] Erasmus MC, Dept Cell Biol, NL-3015 GD Rotterdam, Netherlands

[7] Univ Cologne, Ctr Mol Med Cologne, D-50931 Cologne, Germany

来源：

SCIENCE ADVANCES | 2021年 / 7卷 / 43期

基金：

荷兰研究理事会;

关键词：

HUMAN COHESIN; X-CHROMOSOME; TRANSCRIPTION; ORGANIZATION; LOOPS; DNA; ACTIVATION; DOMAINS; BINDING; GENES;

D O I：

10.1126/sciadv.abg8205

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Mammalian chromosomes are three-dimensional entities shaped by converging and opposing forces. Mitotic cell division induces marked chromosome condensation, but following reentry into the G(1) phase of the cell cycle, chromosomes reestablish their interphase organization. Here, we tested the role of RNA polymerase II (RNAPII) in this transition using a cell line that allows its auxin-mediated degradation. In situ Hi-C showed that RNAPII is required for both compartment and loop establishment following mitosis. RNAPs often counteract loop extrusion, and in their absence, longer and more prominent loops arose. Evidence from chromatin binding, super-resolution imaging, and in silico modeling allude to these effects being a result of RNAPII-mediated cohesin loading upon G(1) reentry. Our findings reconcile the role of RNAPII in gene expression with that in chromatin architecture.

引用

页数：13

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