Ti3C2Tx MXene loaded with indocyanine green for synergistic photothermal and photodynamic therapy for drug-resistant bacterium

Infections caused by antibiotic-resistant bacteria are a critical threat to human health. Considering the difficulties and time-consuming nature of synthesizing new antibiotics, it is of great significance and importance to develop the antibiotic-independent antibacterial approaches against drug-resistant bacteria. Nanomaterials-based photothermal therapy (PTT) and photodynamic therapy (PDT) have attracted much attention due to their broadspectrum bactericidal activity, low toxicity, and drug-free feature. In this work, we loaded indocyanine green (ICG) on the Ti3C2Tx MXene nanosheets (454 nm) so as to combine the photothermal effect of MXene with the photodynamic effect of ICG. Without near-infrared (NIR) irradiation, MXene (20 mu g/mL), ICG (5 mu g/mL) or ICGloaded MXene (ICG-MXene) showed no significant antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA). Under NIR, however, the viability loss of MRSA remarkably increased to 45% for MXene, 66% for ICG and 100% for ICG-MXene. We further found that the great anti-MRSA activity of ICG-MXene under NIR was attributed to the combination of photothermal effect of MXene (high temperature) and photodynamic effect of ICG (high level of reactive oxygen species). Our findings indicate that MXene can be used as both the photothermal agent and the carrier of photosensitizers to achieve the synergistic PTT/PDT therapy for bacterial infections.