Coil-globule structure transition and binding characteristics of DNA molecules induced by isoquinoline-based photoactive ionic liquid surfactant

被引：11

作者：

Zhu, Panpan ^{[1
]}

Ding, Yuanhua ^{[1
]}

Guo, Rong ^{[1
]}

机构：

[1] Yangzhou Univ, Sch Chem & Chem Engn, Yangzhou 225002, Jiangsu, Peoples R China

来源：

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS | 2017年 / 531卷

基金：

中国国家自然科学基金;

关键词：

Binding mode; Structural transition; Driving force; Photoactive ionic liquid surfactant; DNA; BOVINE SERUM-ALBUMIN; SINGLET OXYGEN GENERATION; DYNAMIC LIGHT-SCATTERING; CALF THYMUS-DNA; GENE DELIVERY; PHYSICOCHEMICAL CHARACTERIZATION; CATIONIC SURFACTANTS; GROOVE BINDING; MECHANISM; THERAPY;

D O I：

10.1016/j.colsurfa.2017.07.082

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The photodynamic therapy and gene transportation across cell membrane are closely related with the binding behavior of the photoactive vectors on DNA. Here, the binding characteristics of an isoquinoline-based photoactive ionic liquid surfactant, lauryl isoquinolinium bromide ([C(12)iQuin] Br), with DNA was investigated for the first time by various analytical techniques. It was found that the DNA molecules are strongly compacted at a low [C(12)iQuin] Br concentration, and then undergo a complete coil-to-globule structure transition upon further addition of [C(12)iQuin] Br, which was verified by cryo-TEM. Based on the H-1 NMR and 2D NOESY spectra, it can be concluded that the photoactive [C(12)iQuin] Br is an AT-specific minor groove binder. At a lower [C(12)iQuin] Br concentration, the isoquinolinium ring of [C(12)iQuin] Br would be arranged facing to the plane of the minor groove of DNA, and the hydrophobic tails of [C(12)iQuin] Br would be arranged parallel to the minor groove. The hydrophobic interaction between the hydrocarbon chains of the bound [C(12)iQuin] Br and DNA bases provides the dominate driving force in the binding process. The unique binding mode of [C(12)iQuin] Br on DNA causes the formation of the new photoactive structures of [C(12)iQuin] Br-DNA complexes, which would promise the novel applications in the photodynamic therapy or the gene transportation.

引用

页码：150 / 163

页数：14

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