The particle size of rice bran is a critical characteristic for the processing and eating quality of rice bran food. After hammer milling and ball milling, the physicochemical properties of rice bran with different particle size (203.33, 101.33, 71.10, 41.80, and 18.90 mu m) were investigated, including hydration properties, antioxidant properties, nutritional properties, and microstructure features. Rice bran with small particle size (D-50 18.90 mu m) exhibited higher water solubility index, antioxidant activities, and gamma-oryzanol and gamma-aminobutyric acid content, and the swelling capacity was 1.37 times that of large size (D-50 203.33 mu m). The insoluble dietary fiber of rice bran converted into soluble dietary fiber after ball milling. Mercury intrusion, scanning electron microscope, Fourier transform infrared spectrometry, and X-ray diffraction showed that as the particle size decreasing, there was an increase in pore area, decrease in porosity, and exposure of hydrophilic groups, breakage of cellulose structure. Practical applications The particle size of rice bran was reduced to superfine powder with D-50 18.9 mu m by ball milling. The hydration properties of rice bran were significantly improved after ball milling. The superfine rice bran powder exhibited high antioxidant activities and nutritional properties.