Fixation of CO2 using the ethylmalonyl-CoA pathway in the photoheterotrophic marine bacterium Dinoroseobacter shibae

被引:25
作者
Bill, Nelli [1 ]
Tomasch, Juergen [2 ]
Riemer, Alexander [1 ]
Mueller, Katrin [1 ]
Kleist, Sarah [1 ]
Schmidt-Hohagen, Kerstin [1 ]
Wagner-Doebler, Irene [2 ]
Schomburg, Dietmar [1 ]
机构
[1] Tech Univ Carolo Wilhelmina Braunschweig, Inst Biochem Biotechnol & Bioinformat, Rebenring 56, D-38106 Braunschweig, Germany
[2] Helmholtz Ctr Infect Res HZI, Dept Microbial Commun, Inhoffenstr 7, D-38124 Braunschweig, Germany
关键词
AEROBIC ANOXYGENIC PHOTOTROPHS; SP-NOV; BIOCONDUCTOR PACKAGE; MULTIPLE REGULATORS; GEN; NOV; LIGHT; BACTERIOCHLOROPHYLL; EXPRESSION; POLYHYDROXYALKANOATE; TRANSHYDROGENASE;
D O I
10.1111/1462-2920.13746
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The ability of aerobic anoxygenic photoheterotrophs (AAPs) to gain additional energy from sunlight represents a competitive advantage, especially in conditions where light has easy access or under environmental conditions may change quickly, such as those in the world ' s oceans. However, the knowledge about the metabolic consequences of aerobic anoxygenic photosynthesis is very limited. Combining transcriptome and metabolome analyses, isotopic labelling techniques, measurements of growth, oxygen uptake rates, flow cytometry, and a number of other biochemical analytical techniques we obtained a comprehensive overview on the complex adaption of the marine bacterium Dinoroseobacter shibae DFL12(T) during transition from heterotrophy to photoheterotrophy (growth on succinate). Growth in light was characterized by reduced respiration, a decreased metabolic flux through the tricarboxylic acid (TCA) cycle and the assimilation of CO2 via an enhanced flux through the ethylmalonyl-CoA (EMC) pathway, which was shown to be connected to the serine metabolism. Adaptation to photoheterotrophy is mainly characterized by metabolic reactions caused by a surplus of reducing potential and might depend on genes located in one operon, encoding branching point enzymes of the EMC pathway, serine metabolism and the TCA cycle.
引用
收藏
页码:2645 / 2660
页数:16
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