Determining UV Inactivation of Toxoplasma gondii Oocysts by Using Cell Culture and a Mouse Bioassay

被引：37
作者：
Ware, Michael W.
Augustine, Swinburne A. J.
Erisman, David O.
See, Mary Jean [3 ]
Wymer, Larry
Hayes, Samuel L. [2 ]
Dubey, J. P. [4 ]
Villegas, Eric N. [1 ,3 ]
机构：
[1] US EPA, Biohazard Assessment Res Branch, Microbiol & Chem Exposure Assessment Res Div, Natl Exposure Res Lab, Cincinnati, OH 45268 USA
[2] US EPA, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA
[3] Univ Cincinnati, McMicken Coll Arts & Sci, Dept Biol Sci, Cincinnati, OH 45220 USA
[4] ARS, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 15期
基金：
美国国家环境保护局;
关键词：
CRYPTOSPORIDIUM-PARVUM OOCYSTS; SEVERE ACQUIRED TOXOPLASMOSIS; LOW-PRESSURE ULTRAVIOLET; ENHYDRA-LUTRIS-NEREIS; REAL-TIME PCR; WATERBORNE TOXOPLASMOSIS; DRINKING-WATER; SEA OTTERS; UNITED-STATES; FRENCH-GUIANA;
D O I：
10.1128/AEM.00153-10
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The effect of UV exposure on Toxoplasma gondii oocysts has not been completely defined for use in water disinfection. This study evaluated UV-irradiated oocysts by three assays: a SCID mouse bioassay, an in vitro T. gondii oocyst plaque (TOP) assay, and a quantitative reverse transcriptase real-time PCR (RT-qPCR) assay. The results from the animal bioassay show that 1- and 3-log(10) inactivation is achieved with 4 mJ/cm(2) UV and 10 mJ/cm(2) low-pressure UV, respectively. TOP assay results, but not RT-qPCR results, correlate well with bioassay results. In conclusion, a 3-log(10) inactivation of T. gondii oocysts is achieved by 10-mJ/cm(2) low-pressure UV, and the in vitro TOP assay is a promising alternative to the mouse bioassay.
引用
收藏
页码：5140 / 5147
页数：8
相关论文
共 64 条
[61]   Evaluation of a strategy for Toxoplasma gondii oocyst detection in water [J].
Villena, I ;
Aubert, D ;
Gomis, P ;
Ferté, H ;
Inglard, JC ;
Denis-Bisiaux, H ;
Dondon, JM ;
Pisano, E ;
Ortis, N ;
Pinon, JM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (07) :4035-4039
[62]   Chemical inactivation of Toxoplasma gondii oocysts in water [J].
Wainwright, Katlyn E. ;
Miller, Melissa A. ;
Barr, Bradd C. ;
Gardner, Ian A. ;
Melli, Ann C. ;
Essert, Tim ;
Packham, Andrea E. ;
Truong, Tin ;
Lagunas-Solar, Manuel ;
Conrad, Patricia A. .
JOURNAL OF PARASITOLOGY, 2007, 93 (04) :925-931
[63]   Physical inactivation of Toxoplasma gondii oocysts in water [J].
Wainwright, Katlyn E. ;
Lagunas-Solar, Manuel ;
Miller, Melissa A. ;
Barr, Bradd C. ;
Gardner, Ian A. ;
Pina, Cecilia ;
Melli, Ann C. ;
Packham, Andrea E. ;
Zeng, Nolan ;
Truong, Tin ;
Conrad, Patricia A. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (17) :5663-5666
[64]   Detection of Toxoplasma gondii Oocysts in Water Sample Concentrates by Real-Time PCR [J].
Yang, Wenli ;
Lindquist, H. D. Alan ;
Cama, Vitaliano ;
Schaefer, Frank W., III ;
Villegas, Eric ;
Fayer, Ronald ;
Lewis, Earl J. ;
Feng, Yaoyu ;
Xiao, Lihua .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (11) :3477-3483
1234567