Determining UV Inactivation of Toxoplasma gondii Oocysts by Using Cell Culture and a Mouse Bioassay

被引：37

作者：

Ware, Michael W.

Augustine, Swinburne A. J.

Erisman, David O.

See, Mary Jean ^{[3
]}

Wymer, Larry

Hayes, Samuel L. ^{[2
]}

Dubey, J. P. ^{[4
]}

Villegas, Eric N. ^{[1
,3
]}

机构：

[1] US EPA, Biohazard Assessment Res Branch, Microbiol & Chem Exposure Assessment Res Div, Natl Exposure Res Lab, Cincinnati, OH 45268 USA

[2] US EPA, Natl Risk Management Res Lab, Cincinnati, OH 45268 USA

[3] Univ Cincinnati, McMicken Coll Arts & Sci, Dept Biol Sci, Cincinnati, OH 45220 USA

[4] ARS, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 15期

基金：

美国国家环境保护局;

关键词：

CRYPTOSPORIDIUM-PARVUM OOCYSTS; SEVERE ACQUIRED TOXOPLASMOSIS; LOW-PRESSURE ULTRAVIOLET; ENHYDRA-LUTRIS-NEREIS; REAL-TIME PCR; WATERBORNE TOXOPLASMOSIS; DRINKING-WATER; SEA OTTERS; UNITED-STATES; FRENCH-GUIANA;

D O I：

10.1128/AEM.00153-10

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of UV exposure on Toxoplasma gondii oocysts has not been completely defined for use in water disinfection. This study evaluated UV-irradiated oocysts by three assays: a SCID mouse bioassay, an in vitro T. gondii oocyst plaque (TOP) assay, and a quantitative reverse transcriptase real-time PCR (RT-qPCR) assay. The results from the animal bioassay show that 1- and 3-log(10) inactivation is achieved with 4 mJ/cm(2) UV and 10 mJ/cm(2) low-pressure UV, respectively. TOP assay results, but not RT-qPCR results, correlate well with bioassay results. In conclusion, a 3-log(10) inactivation of T. gondii oocysts is achieved by 10-mJ/cm(2) low-pressure UV, and the in vitro TOP assay is a promising alternative to the mouse bioassay.

引用

页码：5140 / 5147

页数：8

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