Recent advances in DNA assembly technologies

被引：97

作者：

Chao, Ran ^{[1
]}

Yuan, Yongbo ^{[1
]}

Zhao, Huimin ^{[1
,2
,3
,4
]}

机构：

[1] Univ Illinois, Inst Genom Biol, Dept Chem & Biomol Engn, Urbana, IL 61801 USA

[2] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

[3] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA

[4] Univ Illinois, Dept Bioengn, Urbana, IL 61801 USA

来源：

FEMS YEAST RESEARCH | 2015年 / 15卷 / 01期

基金：

美国国家卫生研究院;

关键词：

metabolic engineering; synthetic biology; pathway construction; pathway optimization; genome synthesis; automation; COMBINATORIAL GENETIC-TRANSFORMATION; CELL-CELL COMMUNICATION; SACCHAROMYCES-CEREVISIAE; HOMOLOGOUS RECOMBINATION; SYNTHETIC BIOLOGY; SIGNALING ELEMENTS; DIRECTED EVOLUTION; METABOLIC PATHWAYS; CHEMICAL-SYNTHESIS; DIRECT CLONING;

D O I：

10.1111/1567-1364.12171

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

DNA assembly is one of the most important foundational technologies for synthetic biology and metabolic engineering. Since the development of the restriction digestion and ligation method in the early 1970s, a significant amount of effort has been devoted to developing better DNA assembly methods with higher efficiency, fidelity, and modularity, as well as simpler and faster protocols. This review will not only summarize the key DNA assembly methods and their recent applications, but also highlight the innovations in assembly schemes and the challenges in automating the DNA assembly methods.

引用

页数：9

共 82 条

[1] BglBricks: A flexible standard for biological part assembly [J].

Anderson J.C. ;

Dueber J.E. ;

Leguia M. ;

Wu G.C. ;

Arkin A.P. ;

Keasling J.D. .

Journal of Biological Engineering, 4 (1)

[2] Counter-selection facilitated plasmid construction by homologous recombination in Saccharomyces cerevisiae [J].

Anderson, PR ;

Haj-Ahmad, Y .

BIOTECHNIQUES, 2003, 35 (04) :692-+

[3] Total Synthesis of a Functional Designer Eukaryotic Chromosome [J].

Annaluru, Narayana ;

Muller, Heloise ;

Mitchell, Leslie A. ;

Ramalingam, Sivaprakash ;

Stracquadanio, Giovanni ;

Richardson, Sarah M. ;

Dymond, Jessica S. ;

Kuang, Zheng ;

Scheifele, Lisa Z. ;

Cooper, Eric M. ;

Cai, Yizhi ;

Zeller, Karen ;

Agmon, Neta ;

Han, Jeffrey S. ;

Hadjithomas, Michalis ;

Tullman, Jennifer ;

Caravelli, Katrina ;

Cirelli, Kimberly ;

Guo, Zheyuan ;

London, Viktoriya ;

Yeluru, Apurva ;

Murugan, Sindurathy ;

Kandavelou, Karthikeyan ;

Agier, Nicolas ;

Fischer, Gilles ;

Yang, Kun ;

Martin, J. Andrew ;

Bilgel, Murat ;

Bohutski, Pavlo ;

Boulier, Kristin M. ;

Capaldo, Brian J. ;

Chang, Joy ;

Charoen, Kristie ;

Choi, Woo Jin ;

Deng, Peter ;

DiCarlo, James E. ;

Doong, Judy ;

Dunn, Jessilyn ;

Feinberg, Jason I. ;

Fernandez, Christopher ;

Floria, Charlotte E. ;

Gladowski, David ;

Hadidi, Pasha ;

Ishizuka, Isabel ;

Jabbari, Javaneh ;

Lau, Calvin Y. L. ;

Lee, Pablo A. ;

Li, Sean ;

Lin, Denise ;

Linder, Matthias E. .

SCIENCE, 2014, 344 (6179) :55-58

[4]

[Anonymous], 2014, REG STAND BIOL PARTS

[5] Development of genetic circuitry exhibiting toggle switch or oscillatory behavior in Escherichia coli [J].

Atkinson, MR ;

Savageau, MA ;

Myers, JT ;

Ninfa, AJ .

CELL, 2003, 113 (05) :597-607

[6] Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids [J].

Bryksin, Anton V. ;

Matsumura, Ichiro .

BIOTECHNIQUES, 2010, 48 (06) :463-465

[7] Design of artificial cell-cell communication using gene and metabolic networks [J].

Bulter, T ;

Lee, SG ;

Woirl, WWC ;

Fung, E ;

Connor, MR ;

Liao, JC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (08) :2299-2304

[8] Refinement and standardization of synthetic biological parts and devices [J].

Canton, Barry ;

Labno, Anna ;

Endy, Drew .

NATURE BIOTECHNOLOGY, 2008, 26 (07) :787-793

[9] R2oDNA Designer: Computational Design of Biologically Neutral Synthetic DNA Sequences [J].

Casini, Arturo ;

Christodoulou, Georgia ;

Freemont, Paul S. ;

Baldwin, Geoff S. ;

Ellis, Tom ;

MacDonald, James T. .

ACS SYNTHETIC BIOLOGY, 2014, 3 (08) :525-528

[10] One-pot DNA construction for synthetic biology: the Modular Overlap-Directed Assembly with Linkers (MODAL) strategy [J].

Casini, Arturo ;

MacDonald, James T. ;

De Jonghe, Joachim ;

Christodoulou, Georgia ;

Freemont, Paul S. ;

Baldwin, Geoff S. ;

Ellis, Tom .

NUCLEIC ACIDS RESEARCH, 2014, 42 (01) :e7

← 1 2 3 4 5 6 7 8 9 →