Determination of non-protein cysteine in human serum by a designed BODIPY-based fluorescent probe

被引：45

作者：

Lu, Jinxin ^{[1
]}

Sun, Chengdong ^{[1
]}

Chen, Wei ^{[1
]}

Ma, Huimin ^{[1
]}

Shi, Wen ^{[1
]}

Li, Xiaohua ^{[1
]}

机构：

[1] Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China

来源：

TALANTA | 2011年 / 83卷 / 03期

关键词：

BODIPY-based derivative; Fluorescent probe; Cysteine detection; Human serum; AMPEROMETRIC DETECTION; TOTAL HOMOCYSTEINE; MASS-SPECTROMETRY; HUMAN PLASMA; THIOLS; GLUTATHIONE; DERIVATIZATION; QUANTIFICATION; ELECTRODE; REAGENT;

D O I：

10.1016/j.talanta.2010.11.023

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel fluorescent probe is designed and synthesized for the determination of cysteine in biological samples by incorporating 2,4-dinitrobenzenesulfonyl (DBS) group as a quencher into the BODIPY skeleton. The BODIPY-based probe itself shows weak fluorescence due to the strong intramolecular charge transfer process. Upon reaction with cysteine, however, the probe produces a rapid and large fluorescence enhancement through the removal of the DBS unit by nucleophilic aromatic substitution. This valuable property leads to the development of a new and simple method for cysteine assay. Under the optimized conditions, the fluorescence enhancement value is directly proportional to the concentration of cysteine in the range 2-12 mu M, with a detection limit of 30 nM (S/N = 3). The applicability of the developed method has been successfully demonstrated on the determination of non-protein cysteine in human serum. Compared to most of the existing fluorescent probes proposed for cysteine, the BODIPY-based one exhibits an excellent overall performance in terms of selectivity, sensitivity and simplicity. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：1050 / 1056

页数：7

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