Recombinant neuritin affects the senescence, apoptosis, proliferation, and migration of rat bone marrow-derived mesenchymal stem cells

被引：8
作者：
Yuan, Wumei [1 ]
Cui, Lijuan [2 ]
Li, Guoxiang [1 ]
Wang, Na [1 ]
Zhang, Pan [1 ]
Zhang, Yunhua [1 ]
Yu, Na [1 ]
Wang, Hongying [1 ]
Zhu, Jingling [1 ]
Yang, Lei [3 ]
Huang, Jin [1 ]
机构：
[1] Shihezi Univ, Sch Med, Dept Biochem, Key Lab Xinjiang Endem & Ethn Dis, Shihezi 832002, Xinjiang, Peoples R China
[2] Shihezi Univ, Med Coll, Affiliated Hosp 1, Shihezi 832008, Xinjiang, Peoples R China
[3] Hangzhou Normal Univ, Sch Med, Hangzhou 310036, Zhejiang, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2017年 / 39卷 / 11期
关键词：
Apoptosis; BMSCs; Cell viability; Migration; Neuritin; Senescence; ACTIVITY-REGULATED GENES; NERVOUS-SYSTEM; EXPRESSION; TRANSPLANTATION; ISCHEMIA; INJURY;
D O I：
10.1007/s10529-017-2411-5
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
To study the effects of recombinant neuritin expressed by Pichia pastoris GS115 on the senescence, apoptosis, proliferation, and migration associated with rat bone marrow-derived mesenchymal stem cells (BMSCs). Recombinant neuritin was purified by Ni-affinity chromatography and identified by western blot and MALDI-TOF spectrometry. The effects of recombinant neuritin on senescence, apoptosis, proliferation, and migration of rat BMSCs WERE investigated. beta-Galactosidase staining indicated that recombinant neuritin administration significantly inhibited BMSCs senescence at 1 mu g neuritin/ml. Additionally, recombinant neuritin reduced the number of apoptotic cells at the early stage according to Annexin V/propidium iodide staining and inhibited cell proliferation according to MTT assay results. Moreover wound healing assay results showed that recombinant neuritin promoted BMSCs migration in the neuritin-treatment group. Recombinant neuritin affects the senescence, apoptosis, proliferation, migration of rat BMSCs. Our findings offer insight into neuritin function outside of the nervous system.
引用
收藏
页码：1649 / 1655
页数：7
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