Crystallization and preliminary X-ray diffraction study of riboflavin synthase

被引：1
|
作者：
Wawrzak, Z
Viitanen, PV
Calabrese, JC
Jordan, DB
机构：
[1] Dupont Co, Cent Res & Dev, Expt Stn, Wilmington, DE 19880 USA
[2] DuPont Pharmaceut Co, Expt Stn, Wilmington, DE 19880 USA
来源：
PROTEIN AND PEPTIDE LETTERS | 2001年 / 8卷 / 01期
关键词：
D O I：
10.2174/0929866013409698
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Escherichia coli riboflavin synthase crystallizes at 22 degreesC in the presence of 7-10% by volume diglyme, 20-50 mM MgCl2 and pH 7.0. In this medium diffraction quality crystals are routinely obtained within 5 h and they are stable for 10 weeks. The crystals are orthogonal in space group P2(1)2(1)2(1) with unit cell dimensions of a = 52.4 Angstrom, b = 62.1 Angstrom, c = 218.8 Angstrom. A 97% complete data set was collected at 2.1 Angstrom resolution.
引用
收藏
页码：69 / 73
页数：5
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