Rapid gene discovery in plant parasitic nematodes via Expressed Sequence Tags

被引：32

作者：

McCarter, J

Abad, P

Jones, JT

Bird, D ^{[1
]}

机构：

[1] N Carolina State Univ, Dept Plant Pathol, Plant Nematode Genet Grp, Raleigh, NC 27695 USA

[2] INRA, Unite Sante Plantes & Environm, F-06606 Antibes, France

[3] Scottish Crop Res Inst, Mycol Bacteriol & Nematol Unit, Dundee DD2 5DA, Scotland

[4] N Carolina State Univ, Dept Plant Pathol, Plant Nematode Genet Grp, Raleigh, NC 27695 USA

来源：

NEMATOLOGY | 2000年 / 2卷

关键词：

Caenorhabditis elegans; clustering; EST; Globodera; Meloidogyne; NemaGene;

D O I：

10.1163/156854100509574

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Projects currently underway are generating thousands of publicly available DNA sequences representing numerous genes from plant parasitic nematodes. Use of these data has the potential to revolutionise gene discovery, as well as aiding in genome physical mapping and expression profiling experiments. This article introduces sequences called expressed sequence tags or ESTs, which are single-sequence reads from randomly-selected cDNA clones. We review the process used to create these sequences and outline the strengths and weaknesses of ESTs as research tools. Instructions on how to access and use EST data also are provided.

引用

页码：719 / 731

页数：13

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