Isolation, Identification, and Antibacterial Mechanisms of Bacillus amyloliquefaciens QSB-6 and Its Effect on Plant Roots

被引:51
作者
Duan, Yanan [1 ]
Chen, Ran [1 ]
Zhang, Rong [1 ]
Jiang, Weitao [1 ]
Chen, Xuesen [1 ]
Yin, Chengmiao [1 ]
Mao, Zhiquan [1 ]
机构
[1] Shandong Agr Univ, Coll Hort Sci & Engn, Natl Key Lab Crop Biol, Shandong, Peoples R China
基金
中国国家自然科学基金;
关键词
apple replant disease; Fusarium spp; Bacillus amyloliquefaciens; metabolites; root; identification; APPLE REPLANT DISEASE; SOIL MICROBIAL COMMUNITIES; BIOLOGICAL-CONTROL; ANTIFUNGAL ACTIVITY; FUSARIUM-OXYSPORUM; IN-VITRO; ANTAGONISTIC BACTERIA; BIOORGANIC FERTILIZER; PHYLOGENETIC ANALYSIS; RHIZOCTONIA-SOLANI;
D O I
10.3389/fmicb.2021.746799
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Apple replant disease (ARD) is a common problem in major apple planting areas, and biological factors play a leading role in its etiology. Here, we isolated the bacterial strain QSB-6 from the rhizosphere soil of healthy apple trees in a replanted orchard using the serial dilution method. Strain QSB-6 was provisionally identified as Bacillus amyloliquefaciens based on its morphology, physiological and biochemical characteristics, carbon source utilization, and chemical sensitivity. Maximum likelihood analysis based on four gene sequences [16S ribosomal RNA gene (16S rDNA), DNA gyrase subunit A (gyrA), DNA gyrase subunit B (gyrB), and RNA polymerase subunit B (rpoB)] from QSB-6 and other strains indicated that it had 100% homology with B. amyloliquefaciens, thereby confirming its identification. Flat standoff tests showed that strain QSB-6 had a strong inhibitory effect on Fusarium proliferatum, Fusarium solani, Fusarium verticillioides, Fusarium oxysporum, Alternaria alternata, Aspergillus flavus, Phoma sp., Valsa mali, Rhizoctonia solani, Penicillium brasilianum, and Albifimbria verrucaria, and it had broad-spectrum antibacterial characteristics. Extracellular metabolites from strain QSB-6 showed a strong inhibitory effect on Fusarium hyphal growth and spore germination, causing irregular swelling, atrophy, rupture, and cytoplasmic leakage of fungal hyphae. Analysis of its metabolites showed that 1,2-benzenedicarboxylic acid and benzeneacetic acid, 3- hydroxy-, methyl ester had good inhibitory effects on Fusarium, and increased the length of primary roots and the number of lateral roots of Arabidopsis thaliana plantlet. Pot experiments demonstrated that a QSB-6 bacterial fertilizer treatment (T2) significantly improved the growth of Malus hupehensis Rehd. seedlings. It increased root length, surface area, tips, and forks, respiration rate, protective enzyme activities, and the number of soil bacteria while reducing the number of soil fungi. Fermentation broth from strain QSB-6 effectively prevented root damage from Fusarium. terminal restriction fragment length polymorphism (T-RFLP) and quantitative PCR (qPCR) assays showed that the T2 treatment significantly reduced the abundance of Fusarium in the soil and altered the soil fungal community structure. In summary, B. amyloliquefaciens QSB-6 has a good inhibitory effect on Fusarium in the soil and can significantly promote plant root growth. It has great potential as a biological control agent against ARD.
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页数:24
相关论文
共 174 条
[91]   Fusarium rot of melon is caused by several Fusarium species [J].
Medeiros Araujo, Maria B. ;
Moreira, Glaucia M. ;
Nascimento, Luan Vitor ;
Nogueira, Geovane de Almeida ;
Nascimento, Selma Rogeria de C. ;
Pfenning, Ludwig H. ;
Ambrosio, Marcia Michelle de Q. .
PLANT PATHOLOGY, 2021, 70 (03) :712-721
[92]  
Meng Pin-pin, 2012, Yingyong Shengtai Xuebao, V23, P3079
[93]   Evaluating different approaches for the quantification of oomycete apple replant pathogens, and their relationship with seedling growth reductions [J].
Moein, S. ;
Mazzola, M. ;
Spies, C. F. J. ;
McLeod, A. .
EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2019, 154 (02) :243-257
[94]   Effect-Directed Isolation and Identification of Antibacterial Chelidonium majus L. Alkaloids [J].
Moricz, Agnes M. ;
Fornal, Emilia ;
Jesionek, Wioleta ;
Majer-Dziedzic, Barbara ;
Choma, Irena M. .
CHROMATOGRAPHIA, 2015, 78 (9-10) :707-716
[95]  
Nagorska K, 2007, ACTA BIOCHIM POL, V54, P495
[96]   Plant Endophytes and Epiphytes: Burgeoning Sources of Known and "Unknown" Cytotoxic and Antibiotic Agents? [J].
Newman, David J. ;
Cragg, Gordon M. .
PLANTA MEDICA, 2020, 86 (13/14) :891-905
[97]   Reanalysis of microbiomes in soils affected by apple replant disease (ARD): Old foes and novel suspects lead to the proposal of extended model of disease development [J].
Nicola, Lidia ;
Insam, Heribert ;
Pertot, Ilaria ;
Stres, Blaz .
APPLIED SOIL ECOLOGY, 2018, 129 :24-33
[98]   Biocontrol of fungal root rot diseases of crop plants by the use of rhizobia and bradyrhizobia [J].
Omar, SA ;
Abd-Alla, MH .
FOLIA MICROBIOLOGICA, 1998, 43 (04) :431-437
[100]  
Ouhaibi-Ben Abdeljalil N., 2016, Journal of Plant Pathology and Microbiology, V7, P331